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首页> 外文期刊>Biotechnology Progress >Use and optimization of a dual-flowrate loading strategy to maximize throughput in protein-A affinity chromatography
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Use and optimization of a dual-flowrate loading strategy to maximize throughput in protein-A affinity chromatography

机译：使用和优化双流速上样策略以最大程度提高A蛋白亲和色谱的通量

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摘要

The effect of an alternate strategy employing two different flowrates during loading was explored as a means of increasing system productivity in Protein-A chromatography. The effect of such a loading strategy was evaluated using a chromatographic model that was able to accurately predict experimental breakthrough curves for this Protein-A system. A gradient-based optimization routine is carried out to establish the optimal loading conditions (initial and final flowrates and switching time). The two-step loading strategy (using a higher flowrate during the initial stages followed by a lower flowrate) was evaluated for an Fc-fusion protein and was found to result in significant improvements in process throughput. In an extension of this optimization routine, dynamic loading capacity and productivity were simultaneously optimized using a weighted objective function, and this result was compared to that obtained with the single flowrate. Again, the dual-flowrate strategy was found to be superior.
机译：探索了在加载过程中采用两种不同流速的替代策略的效果，作为提高Protein-A色谱系统生产率的一种手段。使用色谱模型评估了这种加载策略的效果，该色谱模型能够准确预测此Protein-A系统的实验突破曲线。执行基于梯度的优化例程以建立最佳加载条件（初始和最终流量以及切换时间）。对Fc融合蛋白评估了两步加载策略（在初始阶段使用较高的流量，然后使用较低的流量），发现该策略可显着提高过程通量。在此优化程序的扩展中，使用加权目标函数同时优化了动态负载能力和生产率，并将此结果与单流量获得的结果进行了比较。同样，发现双流量策略是更好的。

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