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Embryo sHLA-G secretion is related to pregnancy rate

机译：胚胎shla-g分泌物与妊娠率有关

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HLA-G expression has been detected in early preimplantation embryos and it has been postulated that a relationship between embryonic expression of this factor and successful pregnancy may exist. Forty-six patients were prospectively selected from our centre 'Unidad de Reproduccion Humana, Hospital Universitario de Canarias' for conducting this study. In all cases, metaphase II (MII) oocytes were fertilized using intracytoplasmic sperm injection 2-4 h after retrieval. Embryos were cultured individually in 20 mu l droplets of G-1 medium (VitroLife) under oil at 37 degrees C and a 6% CO2 environment. Fertilization was assessed at 18 h postinsemination and all oocytes fertilized were passed into a new culture plaque individually in 300 mu l culture medium until day 3 of culture. The culture medium was examined for the expression and secretion of sHLA-G with a sandwich enzyme-linked immunosorbent assay kit (BioVendor, Heidelberg, Germany) according to the manufacturer's instructions. We found statistical significance between higher levels of sHLA-G secretion and pregnancy rate. When both groups were compared there was no difference in embryo quality of transferred embryos, but a significant difference in the number of oocytes and the embryo quality of the cohort existed that was greater in the pregnant group. A standardized sHLA-G assay with a specifically defined range and standard units provides a non-invasive method to identify the most competent embryos for transfer.
机译：在早期的预催化胚胎中检测到HLA-G表达，并且已经假定了这种因子和成功怀孕的胚胎表达之间的关系。四十六名患者从我们的中心'Unidad de Rocolucion Mumana，医院大学大学德加那加州伊加里亚岛进行了前瞻性，用于进行这项研究。在所有情况下，在检索后使用2-4小时施用中脱发II（MII）卵母细胞。在37摄氏度下在油下单独培养胚胎在20μl的G-1培养基（Vitroge）下培养和6％的CO 2环境。施肥于18小时后评估，施肥的所有卵母细胞都在300μl培养基中单独进入新的培养斑块，直到培养的第3天。根据制造商的说明，检查了用夹心酶联免疫吸附测定套件（Biovendor，Heidelberg，德国）的Shla-g的表达和分泌的培养基。我们发现较高水平的SHLA-G分泌和妊娠率之间的统计学意义。当两组进行比较时，转移胚胎的胚胎质量没有差异，但卵母细胞数量和群组的胚胎质量的显着差异在怀孕组中更大。具有特异性定义范围和标准单元的标准化SHLA-G测定提供了一种非侵入性方法，以识别转移最称重的胚胎。

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