Quantification of Bhas 42 cell transformation assay with hydrogen peroxide method

Kiyoshi Sasaki; Dai Muramatsu; Shoko Arai; Nobuko Endou; Ayako Sakai; Shojiro Yamazaki; Makoto Umeda; Noriho Tanaka

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Quantification of Bhas 42 cell transformation assay with hydrogen peroxide method

机译：过氧化氢法定量Bhas 42细胞转化测定

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In transformation assays, transformation frequency has been quantified by time-consuming and subjective scoring of transformed foci under a microscope. We found that hydrogen peroxide kills specifically normal cells but not transformed foci, and havedeveloped a hydrogen peroxide method to measure transformation frequency in Bhas 42 cells (v-Ha-ras gene transfected BALB/c 3T3 cells). The present study was performed to evaluate the hydrogen peroxide method by comparing with the conventional microscopemethod [1-3] with 21 chemicals (13 carcinogens and eight non-carcinogens).

机译：在转化测定中，通过在显微镜下对转化灶的耗时和主观评分来量化转化频率。我们发现过氧化氢能杀死特定的正常细胞，但不能杀死转化的灶，并开发了一种过氧化氢方法来测量Bhas 42细胞（v-Ha-ras基因转染的BALB / c 3T3细胞）中的转化频率。本研究是通过与传统显微镜方法[1-3]和21种化学药品（13种致癌物和8种非致癌物）进行比较来评估过氧化氢方法。

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《Alternatives to Animal Testing and Experimentation》 |2011年第1期|共1页
作者
Kiyoshi Sasaki; Dai Muramatsu; Shoko Arai; Nobuko Endou; Ayako Sakai; Shojiro Yamazaki; Makoto Umeda; Noriho Tanaka;
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正文语种 eng
中图分类动物学;
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1. Quantification of Bhas 42 cell transformation assay with hydrogen peroxide method [J] . Kiyoshi Sasaki, Dai Muramatsu, Shoko Arai, Alternatives to Animal Testing and Experimentation . 2011,第Suppla1期

机译：过氧化氢法定量Bhas 42细胞转化测定
2. A validation study on the 96-well method of Bhas 42 cell transformation assay [J] . Ayako Sakai, Shoko Arai, Kiyoshi Sasaki, Alternatives to Animal Testing and Experimentation . 2011,第Suppla1期

机译：Bhas 42细胞转化试验96孔方法的验证研究
3. Detection of initiating as well as promoting activity of chemicals by a novel cell transformation assay using v-Ha-ras-transfected BALB/c 3T3 cells (Bhas 42 cells). [J] . Asada S, Sasaki K, Tanaka N, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 2005,第1期

机译：使用v-Ha-ras转染的BALB / c 3T3细胞（Bhas 42细胞）通过新型细胞转化测定法检测化学物质的启动和促进活性。
4. Comparison of Densitometry, Refractive Index, and Classical Permanganate Titration Assay Methods with Commercially Available Rocket-Grade Hydrogen Peroxide [C] . Michael D. McPherson International conference on green propellants for space propulsion . 2004

机译：用市售火箭级氢过氧化氢的密度测定，折射率和经典高锰酸盐滴定法测定方法的比较
5. Mouse mammary tumor virus activates Cdc42 leading to filopodia formation and transformation of mammary epithelial cells [D] . Smith, Gail Perry 2005

机译：小鼠乳腺肿瘤病毒激活Cdc42导致丝状伪足形成和乳腺上皮细胞转化
6. Comparative tumor promotion assessment of e‐cigarette and cigarettes using the in vitro Bhas 42 cell transformation assay [O] . Damien Breheny, Oluwatobiloba Oke, Kamala Pant, -1

机译：使用体外Bhas 42细胞转化测定法对电子烟和香烟的肿瘤促进作用进行比较评估
7. EURL ECVAM Recommendation on the Cell Transformation Assay based on the Bhas 42 cell line [O] . CORVI Raffaella, GRIESINGER Claudius, AMCOFF SVEN PATRIK, 2013

机译：EURL ECVAM关于基于Bhas 42细胞系的细胞转化测定的建议

Quantification of Bhas 42 cell transformation assay with hydrogen peroxide method

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