The optimization for preparing the lymph node cell suspension in LLNA BrdU-ELISA

Yuko Maruyama; Atsuko Yuasa; Takanori Hioki; Toshihiko Kasahara; Hajime Kojima

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The optimization for preparing the lymph node cell suspension in LLNA BrdU-ELISA

机译：LLNA BrdU-ELISA中制备淋巴结细胞悬液的优化

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LLNA BrdU-ELISA was developed as a non-radioisotopic modification of the original LLNA, and added to OECD Guidelines for the Testing Chemicals in 2010. In this assay, the procedure for preparing the suspension of lymph node cells is critical to produce the reliable data. A disposable plastic pestle is commonly used to homogenize the lymph nodes, however, the data may be affected by the operators' skill and operation time. Therefore, we verified the artifacts in this homogenization procedure, and validated the utility of the beads homogenizer for cell disaggregating.

机译：LLNA BrdU-ELISA的开发是对原始LLNA的非放射性同位素修饰，并于2010年添加到OECD测试化学品指南中。在该分析中，制备淋巴结细胞悬液的过程对于产生可靠的数据至关重要。通常使用一次性塑料杵来使淋巴结均匀，但是，数据可能会受到操作人员的技能和手术时间的影响。因此，我们验证了此均质程序中的伪影，并验证了珠均质器在细胞分解中的效用。

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《Alternatives to Animal Testing and Experimentation》 |2011年第1期|共1页
作者
Yuko Maruyama; Atsuko Yuasa; Takanori Hioki; Toshihiko Kasahara; Hajime Kojima;
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正文语种 eng
中图分类动物学;
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1. The optimization for preparing the lymph node cell suspension in LLNA BrdU-ELISA [J] . Yuko Maruyama, Atsuko Yuasa, Takanori Hioki, Alternatives to Animal Testing and Experimentation . 2011,第Suppla1期

机译：LLNA BrdU-ELISA中制备淋巴结细胞悬液的优化
2. Long-acting combination anti-HIV drug suspension enhances and sustains higher drug levels in lymph node cells than in blood cells and plasma [J] . Kraft John C., McConnachie Lisa A., Koehn Josefin, AIDS . 2017,第6期

机译：长效组合抗HIV药物悬浮液可增强并维持淋巴结细胞中的药物水平，而不是血细胞和血浆
3. Exosomes released by melanoma cells prepare sentinel lymph nodes for tumor metastasis. [J] . Hood JL, San RS, Wickline SA Cancer research: The official organ of the American Association for Cancer Research, Inc . 2011,第11期

机译：黑色素瘤细胞释放的外泌体为肿瘤转移准备了前哨淋巴结。
4. Central Lymph Node Metastasis as a Predictor for Lateral Lymph Node Metastasis in Clinically Node-Negative T3 and T4 Papillary Thyroid Carcinoma [C] . Zhe Han, Shuai Xue, Peisong Wang, International Conference on Information Technology in Medicine and Education . 2018

机译：中央淋巴结转移作为临床节点阴性T3和T4乳头状甲状腺癌中侧淋巴结转移的预测因子
5. Insights into the Transcriptional Identities of Lymph Node Stromal Cell Subsets Isolated from Resting and Inflamed Lymph Nodes. [D] . Malhotra, Deepali. 2013

机译：深入了解从静止和发炎的淋巴结中分离出来的淋巴结基质细胞亚群的转录身份。
6. Long-acting combination anti-HIV drug suspension enhances and sustains higher drug levels in lymph node cells than in blood cells and plasma [O] . John C. Kraft, Lisa A. McConnachie, Josefin Koehn, -1

机译：长效联合抗HIV药物悬浮液可增强并维持淋巴结细胞中的药物水平高于血细胞和血浆中的药物水平
7. Exosomes Released by Melanoma Cells Prepare Sentinel Lymph Nodes for Tumor Metastasis [O] . Joshua L. Hood, Roman Susana San, Samuel A. Wickline 2011

机译：由黑色素瘤细胞释放的外泌体为肿瘤转移准备哨兵淋巴结
8. Results of a Local Lymph Node Assay (LLNA) in Mice with a Dye. [R] . 2016

机译：用染料小鼠的局部淋巴结测定（LLNa）的结果。

The optimization for preparing the lymph node cell suspension in LLNA BrdU-ELISA

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