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首页> 外文期刊>Biotechnology Progress >Hydrolysis of nonstarch carbohydrates of wheat-starch effluent for ethanol production
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Hydrolysis of nonstarch carbohydrates of wheat-starch effluent for ethanol production

机译：水解小麦淀粉废水中的非淀粉碳水化合物以生产乙醇

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摘要

A (polysaccharide-rich) waste stream derived from a combined starch and ethanol factory was investigated regarding hydrolysis of the nonstarch carbohydrates for ethanol production. The material was characterized and processed to yield the maximum amount of sugars. The starch fraction was hydrolyzed with amylolytic enzymes, and the resulting fibrous material was separated by filtration. This material, denoted starch-free fibers (SFF), was subjected to heat treatment followed by enzymatic hydrolysis to recover the other major carbohydrate components, namely, cellulose and hemicellulose, in monomeric form. Heat treatment in a microwave oven efficiently solubilized a fraction of these polysaccharides and made the material more accessible to the cellulolytic and hemicellulolytic enzymes used in the subsequent enzymatic hydrolysis. The maximum sugar yield after enzymatic hydrolysis, achieved with pretreatment at 170 degreesC for 40 min, was 34.1 g per 100 g SFF, comprising 12.8 g glucose, 13.9 g xylose and 7.4 g arabinose, corresponding to 66%, 71% and 51% of the theoretical, respectively.
机译：研究了淀粉和乙醇联合工厂的（富含多糖的）废物流，涉及水解非淀粉碳水化合物以生产乙醇的过程​​。对该材料进行表征和加工以产生最大量的糖。用淀粉分解酶水解淀粉级分，并通过过滤分离得到的纤维材料。将该材料（称为无淀粉纤维（SFF））进行热处理，然后进行酶促水解，以回收单体形式的其他主要碳水化合物成分，即纤维素和半纤维素。在微波炉中进行热处理可以有效地溶解一部分这些多糖，并使该材料更易于用于随后的酶水解中的纤维素分解酶和半纤维素分解酶。在170摄氏度下进行预处理40分钟后，酶促水解后的最大糖产量为34.1 g / 100 g SFF，其中包括12.8 g葡萄糖，13.9 g木糖和7.4 g阿拉伯糖，分别占66％，71％和51％理论上分别。

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