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首页> 外文期刊>Alcohol >Short-term selection for acute ethanol tolerance and sensitization from an F2 population derived from the high and low alcohol-sensitive selectively bred rat lines.
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Short-term selection for acute ethanol tolerance and sensitization from an F2 population derived from the high and low alcohol-sensitive selectively bred rat lines.

机译：从高和低酒精敏感性选择性繁殖的大鼠品系的F2群体中进行急性乙醇耐受性和敏化的短期选择。

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Previous studies have identified quantitative trait loci (QTL) in the inbred high and low alcohol-sensitive rat (IHAS1 and ILAS1) strains. The original development of the strains involved selection for ethanol sensitivity based on duration of the loss of the righting reflex (LORR) after a standard dose of ethanol. This paper confirms some of these QTL using a short-term selection procedure based on the difference between the blood ethanol level at LORR and regain of the righting response. An F(2) population of rats was developed by a reciprocal cross of IHAS1 and ILAS1 rats. Selection for five generations was carried out using delta-blood ethanol concentration (dBEC) as the selection trait, where dBEC=BECLR (BEC at loss of righting reflex)-BECRR (BEC at regain of righting reflex). The lines were labeled tolerant (TOL) or sensitive (SENS). Approximately one-third of the offspring for each generation in each line were genotyped using DNA markers that had been previously found to be linked to QTL on chromosomes 1, 2, 5, 12, and 13. By the fifth generation of selection, the lines showed a very large difference in dBEC, BECRR, and duration of LORR; BECLR showed little segregation during the selection, and latency to lose the righting reflex showed none. IHAS allele frequency increased in the SENS line for markers on chromosomes 1, 5, 12, and 13 while ILAS allele frequency increased in the TOL line. These results were in good agreement with the two previous QTL studies. On chromosome 2, the selection resulted in an accumulation of ILAS alleles in both lines. This study provides independent confirmation of the location of QTL on chromosomes 1, 5, 12, and 13 for ethanol sensitivity. It also suggests that genetic differences in duration of LORR are mediated primarily by the dBEC phenotype.
机译：先前的研究已经在近交高和低酒精敏感性大鼠（IHAS1和ILAS1）品系中确定了数量性状基因座（QTL）。菌株的最初开发涉及基于标准剂量乙醇后的扶正反射丧失（LORR）的持续时间来选择乙醇敏感性。本文根据LORR处的血液乙醇水平与恢复扶正反应之间的差异，使用短期选择程序来确认其中一些QTL。通过相互交叉的IHAS1和ILAS1大鼠发展出F（2）种群。使用Delta-blood乙醇浓度（dBEC）作为选择性状进行五代选择，其中dBEC = BECLR（失去对位反射的BEC）-BECRR（恢复对位反射的BEC）。将这些线标记为耐受（TOL）或敏感（SENS）。使用先前发现与染色体1、2、5、12和13上的QTL连锁的DNA标记，对每个品系中每一代后代的大约三分之一进行基因分型。通过第五代选择，品系在dBEC，BECRR和LORR持续时间方面显示出很大的差异; BECLR在选择过程中几乎没有分离，而失去扶正反射的潜伏期也没有。对于染色体1、5、12和13上的标记，IHAS等位基因频率在SENS系中增加，而TOL系中ILAS等位基因频率在增加。这些结果与之前的两个QTL研究非常吻合。在2号染色体上，选择导致两条细胞系中都积累了ILAS等位基因。这项研究对QTL在1号，5号，12号和13号染色体上的乙醇敏感性进行了独立确认。这也表明，LORR持续时间的遗传差异主要由dBEC表型介导。

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