...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Alcohol >Long-term alcohol ingestion alters the folate-binding kinetics in intestinal brush border membrane in experimental alcoholism.
【24h】

Long-term alcohol ingestion alters the folate-binding kinetics in intestinal brush border membrane in experimental alcoholism.

机译：长期饮酒会改变实验性酒精中毒时肠道刷状缘膜的叶酸结合动力学。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The folic acid transport across epithelial cell membrane of the intestine is an essential step for its absorption, conservation, and homeostasis in the body. In this study, we sought to examine the kinetics of binding to intestinal brush border membrane (BBM) considering intestinal malabsorption as the major contributing factor to alcohol-induced folate deficiency. Male Wistar rats were fed 1g/kg body weight/day ethanol (20% solution) orally for 3 months. We studied [(3)H]-folic acid binding to the intestinal BBM and acidic pH-dependent binding was observed to be associated with reduced maximal binding (B(max)) in chronic ethanol-fed group. However, under such conditions, there was no significant effect of ethanol ingestion on K(d) and pH optimum of the binding process. Increasing the osmolarity at pH 5.5 had no effect on the binding of folate to BBM, thus confirming that the observed changes in B(max) values were due to site-specific binding to the extravesicular sites. Importantly, ethanol ingestion disturbs the S-S status at the binding site besides interfering with the Na(+) and divalent cation dependency of the binding process. These results highlight the possible mechanism of folate malabsorption at primary absorptive site during alcoholism.
机译：叶酸穿过肠道上皮细胞膜的运输是其在体内吸收，保存和体内稳态的重要步骤。在这项研究中，我们试图检查与肠道刷状缘膜（BBM）结合的动力学，其中肠道吸收不良是导致酒精引起的叶酸缺乏的主要因素。给雄性Wistar大鼠口服1g / kg体重/天的乙醇（20％溶液），持续3个月。我们研究了[（3）H]-叶酸与肠道BBM的结合，并观察到酸性pH依赖性结合与慢性乙醇喂养组最大结合减少（B（max））有关。但是，在这种条件下，乙醇摄入对结合过程的K（d）和最适pH值没有显着影响。在pH 5.5下增加渗透压对叶酸与BBM的结合没有影响，因此证实观察到的B（max）值的变化是由于位点特异性结合到囊泡外。重要的是，乙醇的摄入除了干扰结合过程的Na（+）和二价阳离子依赖性之外，还破坏了结合位点的S-S状态。这些结果强调了酒精中毒期间叶酸吸收不良的主要机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号