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首页> 外文期刊>Alcohol >Protein expression changes in the nucleus accumbens and amygdala of inbred alcohol-preferring rats given either continuous or scheduled access to ethanol.
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Protein expression changes in the nucleus accumbens and amygdala of inbred alcohol-preferring rats given either continuous or scheduled access to ethanol.

机译：连续或计划使用乙醇的偏爱酒精的近亲大鼠伏隔核和杏仁核中的蛋白质表达发生变化。

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摘要

Chronic ethanol (EtOH) drinking produces neuronal alterations within the limbic system. To investigate changes in protein expression levels associated with EtOH drinking, inbred alcohol-preferring (iP) rats were given one of three EtOH access conditions in their home-cages: continuous ethanol (CE: 24h/day, 7days/week access to EtOH), multiple scheduled access (MSA: four 1-h sessions during the dark cycle/day, 5 days/week) to EtOH, or remained EtOH-naive. Both MSA and CE groups consumed between 6 and 6.5g of EtOH/kg/day after the 3rd week of access. On the first day of EtOH access for the seventh week, access was terminated at the end of the fourth MSA session for MSA rats and the corresponding time point (2300h) for CE rats. Ten h later, the rats were decapitated, brains extracted, the nucleus accumbens (NAcc) and amygdala (AMYG) microdissected, and protein isolated for 2-dimensional gel electrophoretic analyses. In the NAcc, MSA altered expression levels for 12 of the 14 identified proteins, compared with controls, with six of these proteins altered by CE access, as well. In the AMYG, CE access changed expression levels for 22 of the 27 identified proteins, compared with controls, with 8 of these proteins altered by MSA, as well. The proteins could be grouped into functional categories of chaperones, cytoskeleton, intracellular communication, membrane transport, metabolism, energy production, or neurotransmission. Overall, it appears that EtOH drinking and the conditions under which EtOH is consumed, differentially affect protein expression levels between the NAcc and AMYG. This may reflect differences in neuroanatomical and/or functional characteristics associated with EtOH self-administration and possibly withdrawal, between these two brain structures.
机译：慢性乙醇（EtOH）饮用会在边缘系统内产生神经元改变。为了研究与喝EtOH相关的蛋白质表达水平的变化，向偏爱近交酒精（iP）的大鼠在其家笼中提供了三种EtOH访问条件之一：连续乙醇（CE：每天24小时，每天7天访问EtOH） ，对EtOH的多次预定访问（MSA：在黑暗周期/天，5天/周内进行了四个1-h会话），或者仍然处于未使用过EtOH的状态。进入第3周后，MSA和CE组的EtOH / kg /天摄入量在6到6.5g之间。在第7周的EtOH进入的第一天，对于MSA大鼠，在第四次MSA疗程结束时终止进入，对于CE大鼠，在相应的时间点（2300h）终止。十小时后，将大鼠断头，取出大脑，解剖伏隔核（NAcc）和杏仁核（AMYG），并分离蛋白质进行二维凝胶电泳分析。与对照相比，在NAcc中，MSA改变了14种已鉴定蛋白质中12种的表达水平，其中6种蛋白质也因CE进入而改变。与对照组相比，在AMYG中，CE通路改变了27种已鉴定蛋白质中22种的表达水平，其中8种蛋白质也被MSA改变了。这些蛋白可以分为伴侣蛋白，细胞骨架，细胞内通讯，膜运输，代谢，能量产生或神经传递的功能类别。总体而言，看来饮用EtOH和消耗EtOH的条件会差异影响NAcc和AMYG之间的蛋白质表达水平。这可能反映了这两个大脑结构之间与EtOH自我给药相关的神经解剖学和/或功能特征的差异，并可能退出。

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