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首页> 外文期刊>Diseases of Aquatic Organisms >Effectiveness of different vaccine formulations against vibriosis causedby Vibrio vulnificus serovar E (biotype 2) in European eels Anguillaanguilla
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Effectiveness of different vaccine formulations against vibriosis causedby Vibrio vulnificus serovar E (biotype 2) in European eels Anguillaanguilla

机译：不同疫苗配方对欧洲鳗鱼Anguillaangula的不同疫苗配方导致振动率的血管源性

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摘要

Vibriosis due to Vibrio vulnificus serovar E (biotype 2) is one of the main causes of mortality in European eels cultured in Europe. The main objective of this study was to develop a vaccine and a vaccination procedure against this pathogen. With this aim, we tested several vaccine formulations (inactivated whole-cells with and without toxoids-inactivated extracellular products-from capsulated and uncapsulated strains, attenuated live vaccines and purified lipopolysaccharide [LPS]) on eels maintained under controlled laboratory conditions using different delivery routes (injection and immersion). To study the immune response we estimated antibody titers and bactericidal/bacteriostatic activity in mucus and serum. To evaluate protection, we calculated the relative percent survival (RPS) after intraperitoneal (i.p.) injection and bath challenge of the pathogen. The overall results indicate that: (1) capsular antigens seem to be essential for protective immunization; (2) vaccines confer the highest protection when administered by i.p. injection; (3) booster is needed to achieve good protection by immersion; (4) enriching the vaccine with toxoids enhances protection to optimal levels (RPS values around 70 to 100 %, depending on the delivery route); and (5) the protective effect in serum and mucus depends on the route of administration and seems to be related to the production of specific antibodies.
机译：由于vibrioVibriafificusserovar E（Biotype 2）引起的振动症是欧洲培养的欧洲鳗鱼死亡率的主要原因之一。本研究的主要目的是对该病原体进行疫苗和疫苗接种程序。通过这种目标，我们测试了几种疫苗制剂（灭活的全细胞，没有毒素 - 灭活的细胞外产物 - 从囊泡和未包封的菌株，减毒的活疫苗和纯化的脂多糖[LPS]）在使用不同的递送途径维持在受控实验室条件下维持的鳗鱼（注射和浸没）。研究免疫应答，我们估计粘液和血清中的抗体滴度和杀菌/抑菌活性。为了评估保护，我们计算腹膜内（I.P.）注射和病原体的浴挑战后相对百分比存活（RPS）。总体结果表明：（1）荚膜抗原似乎是保护性免疫的必要性; （2）疫苗在I.P施用时赋予最高保护。注射; （3）助推器需要浸没来实现良好的保护; （4）用毒素富集疫苗提高了对最佳水平的保护（RPS值约为70％至100％，具体取决于递送路线）; （5）血清和粘液的保护作用取决于给药途径，似乎与特定抗体的产生有关。

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