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首页> 外文期刊>Developmental cell >Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative Potential
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Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative Potential

机译：静止细胞主动补充CENP-A核体以维持CENTROMERE身份和增殖性潜力

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摘要

Centromeres provide a robust model for epigenetic inheritance as they are specified by sequence-independent mechanisms involving the histone H3-variant centromere protein A (CENP-A). Prevailing models indicate that the high intrinsic stability of CENP-A nucleosomes maintains centromere identity indefinitely. Here, we demonstrate that CENP-A is not stable at centromeres but is instead gradually and continuously incorporated in quiescent cells including G0-arrested tissue culture cells and prophase I-arrested oocytes. Quiescent CENP-A incorporation involves the canonical CENP-A deposition machinery but displays distinct requirements from cell cycle-dependent deposition. We demonstrate that Plk1 is required specifically for G1 CENP-A deposition, whereas transcription promotes CENP-A incorporation in quiescent oocytes. Preventing CENP-A deposition during quiescence results in significantly reduced CENP-A levels and perturbs chromosome segregation following the resumption of cell division. In contrast to quiescent cells, terminally differentiated cells fail to maintain CENP-A levels. Our work reveals that quiescent cells actively maintain centromere identity providing an indicator of proliferative potential.
机译：Centromeres为表观遗传遗传提供了一种强大的模型，因为它们由涉及组蛋白H3-变体Centromere蛋白A（CENP-A）的序列无关机制。普遍的模型表明CENP-A核体的高固有稳定性无限期地保持了CENTROMERE IDETITY。在这里，我们证明CENP-A在焦粒子下不稳定，而是逐渐和连续地掺入包括G0被捕的组织培养细胞和预先捕获的卵母细胞的静态细胞中。静止CENP-A Contronall涉及规范CENP-A沉积机械，但从细胞周期依赖性沉积中显示出不同的要求。我们证明PLK1专门用于G1 CENP-A沉积，而转录促进CENP-in掺入静止的卵母细胞。在恢复细胞分裂后，在静脉期间防止CENP-A沉积导致显着降低的CENP-A水平和Perturbs染色体隔离。与静态细胞相反，终端分化的细胞不能维持CENP-A水平。我们的工作揭示了静态细胞积极维持Centromere身份，提供增殖潜力的指标。

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