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首页> 外文期刊>Biotechnology Progress >Improved paclitaxel and baccatin III production in suspension cultures of taxus media
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Improved paclitaxel and baccatin III production in suspension cultures of taxus media

机译：在红豆杉培养基悬浮培养中提高紫杉醇和浆果赤霉素III的产量

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A cell suspension cultue of Taxus media was established from a stable callus line of this species.The growth rate and production of paclitaxel and baccatin III of this cell suspension were significantly increased during the shake flask culture in its respective optimum media for cell growth and product formation,which were selectd after assaying 24 different culture media.The highest yields of paclitaxel (2.09 mg L~(-1)and baccatin III(2.56 mg L~(-1) in the production mediumrose (factors of 7.0 and 3.0,respectively) in the presence of methyljasmonate (220 #mu#g g~(-1) FW).When the elicitor was added together with mevalonate (0.38 mM) and N-benzoylglycine (0.2 mM),the increasein the yields of paclitaxel and baccatin III was evenhigher (factors of 8.3 and 4.0 ,respectivley).Thereafter,a two-stage culture for cell suspension was carried out using a 5-1 stirred bioreactor running for 36 days,the first stage being in the cell growth medium until cells entered theirstationary growth phase (12 days) and the second stage being in the production medium supplemented with the elicitor and tow putative precursors in the concentrations indicated above.Under these conditions,21.12 mg L~(-1) of paclitaxel and 56.03 mg L~(-1) of baccatin III were obtained after 8 days of culture in the production medium.
机译：从该物种的稳定愈伤组织中建立了红豆杉培养基的细胞悬浮培养物，在摇瓶培养中，在各自的最佳细胞生长和产物培养基中，该细胞悬浮液的生长速率以及紫杉醇和浆果赤霉素III的产量和产量均显着增加。在24种不同的培养基中进行分析后选择形成的青霉素。生产培养基中紫杉醇（2.09 mg L〜（-1）和浆果赤霉素III（2.56 mg L〜（-1））的最高产量分别上升（分别为7.0和3.0的因子）。 ）在茉莉酸甲酯（220＃mu＃gg〜（-1）FW）存在下。当引发剂与甲羟戊酸（0.38 mM）和N-苯甲酰甘氨酸（0.2 mM）一起添加时，紫杉醇和浆果赤霉素III的产量增加甚至更高（分别为8.3和4.0的因子）。此后​​，使用5-1搅拌的生物反应器进行细胞悬浮液的两阶段培养，运行36天，第一阶段在细胞生长培养基中直至细胞进入静止状态成长阶段（1 2天），第二阶段是在生产培养基中补充上述浓度的引发剂和推定的前体。在这些条件下，21.12 mg L〜（-1）的紫杉醇和56.03 mg L〜（-1）的紫杉醇在生产培养基中培养8天后获得浆果赤霉素III。

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