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首页> 外文期刊>Biotechnology Progress >Lightpdirected simultaneous synthesis of oligopeptides on microarray substrate using a photogenerated acid
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Lightpdirected simultaneous synthesis of oligopeptides on microarray substrate using a photogenerated acid

机译：使用光生酸在微阵列底物上光定向同时合成寡肽

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Photogenerated acid (PGA) was used as the acid to remove the protection group from amino acids or peptide oligomers.Comparative study of the deprotection using a PGA,trisarylsulfonium antimonyhexafluoride (SSb),and trifluoroacetic acid (TFA) was performed on glass microscope slides.The results showed that PGA can replace TFA in the deprotection step of oligopeptide synthesis with comparable efficiencies.Acids needed for the deprotection step were generated insitu by light activiation of the precursor moelcule on the microwell substrae.A maskless laser light illumination system was sued to activate the precursor.The accuracy of the amino acid sequence of the synthesized oligopeptide and the location of the synthesis was illustrated by the specific recognition binding of two differen tmodels;lead(II) ion-peptide biosensor for lead (II) and humanprotein p53 mouse MAb DO1.After parallel pattern,specific binding treatment,and fluorescnece labeling,the fluorescence emission images of the oligopeptide microarray showed fluorescence intensity as a result of specific binding at the correct locations of the array.The stepwise synthesis efficiencies of pentapeptide synthesis on the microwell substrate range are approx 96-100% and do not decrease with respect to the chain length of the peptide.
机译：以光生酸（PGA）为酸，从氨基酸或肽低聚物上除去保护基。在玻璃显微镜载玻片上进行了PGA，三芳基锑六氟锑（SSb）和三氟乙酸（TFA）脱保护的比较研究。结果表明，PGA可以在寡肽合成的去保护步骤中替代TFA，具有相当的效率。去保护步骤所需的酸是通过微孔底物上的前体分子的光激活原位产生的。无掩模激光照明系统被激活合成的寡肽氨基酸序列的准确性和合成的位置通过两种不同tmodels的特异性识别结合来说明;这两种模型分别是铅（II）的铅离子肽生物传感器和人蛋白p53小鼠单克隆抗体DO1。经过平行图案，特异性结合处理和荧光标记后，寡核苷酸的荧光发射图像五肽微阵列由于在阵列的正确位置特异性结合而显示出荧光强度。五肽合成在微孔底物范围内的逐步合成效率约为96-100％，相对于肽链长度而言不会降低。

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