• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Influence of fungal elicitors on production of ajmalicine by cell cultures of Catharanthus roseus
【24h】

Influence of fungal elicitors on production of ajmalicine by cell cultures of Catharanthus roseus

机译：真菌诱导剂对玫瑰花长春花细胞培养物对菊酯生产的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Suspension cultures of Catharanthus roseus (C. roseus) were elicited with fungal cell wall fragments of Aspergillus niger (A. niger), Fusarium moniliforme (F. moniliforme), and Trichoderma viride (T. viride). The effects, of elicitor dosage, exposures time, and age of subculture on ajmalicine accumulation were studied. A higher concentration of elicitor extract responded positively to C. roseus suspension cultures. Ajmalicine accumulation increased by about 3-fold when cells were treated with A. niger, F. moniliforme, and T. viride. The maximum ajmalicine production (75 mug g(-1) dry weight (DW)) was observed in cells treated with T. viride. Cell cultures were elicited with 5% preparation of A. niger, F. moniliforme, and T. viride and exposed for 24, 48,,72, and 96 h. for elicitation. Suspension cultures elicited with T viride for 48 h showed a 3-fold increase (87 mu g(-1) DW) in ajmalicine contents, whereas A. niger and F. moniliforme synthesized a 2-fold increase in alkaloid and yielded 52 and 56 mug g(-1) DW ajmalicine, respectively. C. roseus cells of different age (5,10, 15, 2:0, and 25 days old) were treated with a 5% elicitor of A. niger, F. moniliforme, and T. viride and investigated elicitors activity at different age of cell cultures. Maximum yield 166 mug g(-1) DW of ajmalicine was synthesized in 20 day old suspension cultures treated with T. viride. A longer period of incubation of cell cultures with elicitors adversely affected the ajmalicine synthesis.
机译：用黑曲霉（A. niger），镰孢镰刀菌（F. moniliforme）和木霉菌（Trichoderma viride）（T. viride）的真菌细胞壁片段引发长春花（C. roseus）的悬浮培养。研究了引发剂剂量，暴露时间和传代培养年龄对杜鹃花累积量的影响。较高浓度的激发子提取物对玫瑰色念珠菌悬浮培养物呈阳性反应。当用黑曲霉，念珠状曲霉和T. viride处理细胞时，苯丙胺类药物的积累增加了约3倍。在用T. viride处理的细胞中观察到最大的杜鹃花生产量（75马克杯g（-1）干重（DW））。用5％的黑曲霉，念珠状曲霉和T.viride制备细胞培养物，并暴露24、48、72和96小时。启发。悬浮病毒培养引起的48 h苦艾菊素含量增加了3倍（87μg（-1）DW），而曲霉A. niger和F. moniliforme合成了2倍增加的生物碱，产量分别为52和56分别杯g（-1）DW阿马利辛。用5％的黑曲霉，念珠菌和T. viride激发子处理不同年龄（5、10、15、2：0和25天大的玫瑰色念珠菌细胞），并研究不同年龄的激发子活性细胞培养。在经天青霉处理的20天龄悬浮培养物中合成了最大产量166马克g（-1）DJ的阿马利菊酯。细胞培养物与激发子一起孵育的时间较长，会影响阿马利辛的合成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号