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首页> 外文期刊>Biotechnology Progress >Xylitol Production from Sugarcane Bagasse Hydrolyzate in Fluidized Bed Reactor. Effect of Air Flowrate
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Xylitol Production from Sugarcane Bagasse Hydrolyzate in Fluidized Bed Reactor. Effect of Air Flowrate

机译：在流化床反应器中由甘蔗蔗渣水解产物生产木糖醇。空气流量的影响

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摘要

Cells of Candida guillermondii immobilized onto porous glass spheres were cultured batchwise in a fluidized bed bioreactor for xylitol production from sugarcane bagasse hemicellulose hydrolyzate. An aeration rate of only 25 mL/min ensured minimum yields of xylose consumption (0.60) and biomass production (0.14 gDM/gXyl), as well as maximum xyltiol yield (0.54 gXyt/gXyl) and ratio of immobilized to total cells (0.83). These results suggest that cell metabolism, although slow because of oxygen limitation, was mainly addressed to xylitol production. A progressive increase in the aeration rate up to 140 mL/min accelerated both xylose consumption (from 0.36 to 0.78 gXyl/L·) and xylitol formation (form 0.19 to 0.28 gXyt/L·h) but caused the fractio nof immobilized to total cells and the xyltiol yield to decrease up to 0.22 and 0.36 gXyt/gXyl, respectively. The highest xylitol concentration (17.0 gXyt/L) was obtained at 70 mL/min, but the specific xyltiol productivity and the xyltiol yield were 43% and 22% lower than the corresponding values obtianed at the formed products were used in material balances to evaluate the xylose fractions consumed by C. guilliermondii for xylitol production, complete oxidation through the hexoge monophosphate shunt, and cell growth. The experimental data collected at variable oxygen level allowed estimating a P/O ratio of 1.35 mol_(ATP)/mol_O and overall ATP requirements for biomass growth and maintenance of 3.4 mol_(ATP)/C-mol_DM.
机译：在流化床生物反应器中分批培养固定在多孔玻璃球上的吉氏念珠菌的细胞，以由甘蔗渣半纤维素水解产物生产木糖醇。仅25 mL / min的充气速率可确保最低的木糖消耗量（0.60）和生物量生产（0.14 gDM / gXyl），以及最大的木糖醇产率（0.54 gXyt / gXyl）和固定化细胞与总细胞的比率（0.83） 。这些结果表明，尽管细胞代谢由于氧限制而缓慢，但主要涉及木糖醇的产生。曝气速率逐渐提高至140 mL / min既加快了木糖的消耗（从0.36 gXyl / L·到0.78 gXyl / L·），又加快了木糖醇的形成（从0.19 gXyt / L·h形成），但导致分馏酶固定在总细胞并且二甲苯醇的收率分别降低至0.22和0.36 gXyt / gXyl。在70 mL / min时获得最高的木糖醇浓度（17.0 gXyt / L），但比木糖醇的生产率和木糖醇收率分别比成型产品中规定的相应值低43％和22％。居里氏梭菌（C. guilliermondii）消耗的木糖馏分，用于生产木糖醇，通过六磷酸单磷酸盐分流器完全氧化，以及细胞生长。在可变氧水平下收集的实验数据允许估计P / O比为1.35 mol_（ATP）/ mol_O，而总的ATP需求为生物量生长和维持3.4 mol_（ATP）/ C-mol_DM。

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