Universtanding the metabilic and regulatory pathways of thepatocytes is important for biotchnoloibicl applications involving liver cells includig the development of hepartocyts meatbolic flux analsysis (MFA) was applied to elucidate the changes in intracellular pathway fluxes of primary rat hepatocytes exposed to human plasma and to provide a comprehensive snapshot of the hepatic metabolic profile In the current study the combination of preconditioning and plasma suplementation produced distinct metabilic states.Combining te metabolic flux distribution obtained by MFA with methodologices such as fisher discriminant analysis (FDA) and partial least squares or projection to latent structures (PLS) provided instights into the underlying structure and acusal relationshipo within the data With the aid of these analyses patterns in the cellular response of the hepatocytes that contributed to the separation of the different hepatic states were indenfified.Of particular interest was the recognition of distal pathways that strongly correlated with a particular hepatioc function.The hepatic function investigated were intracellular triglyceride accumulation and urea production.This study illstates a framework for optimizing heptic function and a possibility of of identifying potential targets for improving hepatic functions.
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