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首页> 外文期刊>Biotechnology Progress >Highly Multilayered Urease Decomposes Highly Concentrated Urea
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Highly Multilayered Urease Decomposes Highly Concentrated Urea

机译：高度多层的尿素酶分解高浓度的尿素

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摘要

Urease was immobilized at a density of 1.2 g of urease per gram of a matrix via ion-exchange binding of urease to an anion-exchange polymer chain grafted onto a pore surface of a poruns hollow-fiber membrane ane subsequent cross-liking of ureade with transglutaminase.Urea was hydrolyzed durign the jpermeation of urea solution,the concentration of which ranged from 2 to 8 M,through the pores of the resultant membrane with a thickness of approximately 1 mm.Quantitative hydrolysis of 4 M urea was achieved at a permeation rate lower than 1 mL/h,i.e,a residence time longer than 5.1 min,at ambient temperature.This performance is ascribed to convective transport of urea through the pores rimmed by the urease-immobilized polymer chains at a high density Urease was denatured in the presence of urea at xoncentrations higher than 6 M whil edydrolyzing urea.
机译：脲酶通过尿素酶与嫁接到poruns中空纤维膜孔表面的阴离子交换聚合物链上的离子交换结合而固定在每克基质中的尿素酶浓度为1.2克，随后尿素与在尿素溶液的渗透过程中，尿素被水解，其浓度范围为2至8 M，通过厚度约为1 mm的膜孔进入。在渗透速率下实现了4 M尿素的定量水解低于1 mL / h，即在环境温度下的停留时间长于5.1分钟。该性能归因于尿素通过高密度固定在脲酶固定的聚合物链边缘的孔中的对流传输。 Xoncentrations高于6 M的尿素，而尿素水解。

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