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首页> 外文期刊>Biotechnology Progress >Long-term mechanical and biological stability of an immobilized cell reactor for continuous mixed-strain mesophilic lactic starter production in whey permeate
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Long-term mechanical and biological stability of an immobilized cell reactor for continuous mixed-strain mesophilic lactic starter production in whey permeate

机译：固定式细胞反应器在乳清渗透液中连续混合菌株嗜温乳酸发酵剂生产的长期机械和生物学稳定性

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摘要

Biological and rheological stability of continuous immobilized cell fermentation for the production of mixed-strain mesophilic starters were characterized. Three strains of Lactococcus were immobilized separately in kappa-carrageenan/locust bean gumgel beads. Continuous fermentation was carried out in a 1-litre pH-controlled stirred-tank reactor, operated at 30oC, pH 6.2, and D=2/h in whey permeate medium supplemented with yeast extract (1.5%) and 0.1 M KCl and inoculated with 30% (v/v) bead inoculum (strain ratio 1:1:1). The continuous mixed-strain immobilized cell fermentation had a high biological stability, and no strain became dominant or was eliminated during the 52-day fermentation. The total and specific free cell populations showed hightime stability. All immobilized populations, except MD, were unchanged, but a cross contamination of gel beads initially immobilizing a pure culture occurred, leading to a redistribution of the immobilized population in individual beads. After initial modifications of bead rheological properties during colonization batches, the beads demonstrated a high mechanical stability, even with reduced KCl supplementation of the medium, in the range 0.1-0 M. This work emphasizes the potential of immobilized celltechnology for producing mixed lactic starters in continuous fermentation.
机译：表征了用于生产混合菌株的嗜温发酵剂的连续固定细胞发酵的生物学和流变稳定性。将三株乳球菌分别固定在κ-角叉菜胶/刺槐豆胶凝胶珠中。在1升pH控制的搅拌釜反应器中进行连续发酵，该反应器在补充有酵母提取物（1.5％）和0.1 M KCl的乳清渗透液中，在30oC，pH 6.2和D = 2 / h下操作，并接种30％（v / v）珠子接种物（应变比1：1：1）。连续混合菌株固定化细胞发酵具有很高的生物稳定性，并且在52天的发酵过程中没有菌株成为优势菌株或被淘汰。总的和特定的游离细胞群体显示出高时间稳定性。除MD以外，所有固定化种群均未改变，但最初固定纯培养物的凝胶珠发生交叉污染，从而导致固定化种群在单个珠子中重新分布。在定居批次期间对珠子的流变特性进行初步修改后，即使在减少的KCl补充量（0.1-0 M）下，珠子也表现出很高的机械稳定性。这项工作强调了固定化细胞技术在生产混合乳酸发酵剂中的潜力。连续发酵。

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