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首页> 外文期刊>Biotechnology Progress >Lipase production by Acinetobacter radioresistens in the presence of anonwoven fabric
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Lipase production by Acinetobacter radioresistens in the presence of anonwoven fabric

机译：在非织造布的存在下不动杆菌产生的脂肪酶抵抗力

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Lipase production by Acinetobacter radioresistens was performed in a 2-L tank fermenter equipped with a nonwoven fabric, which was made of nylon 6 fiber and coated with a hydrophobic acrylic resin. The fermentation medium contained 2% (v/v) n-hexadecane as the carbon source. The use of the nonwoven fabric was intended for the dispersion of hydrocarbons; thus, the contact surface for the cells to assimilate n-hexadecane can be increased without using emulsifiers. The formation of lipase was found to be growth-associated when the cells grew on n-hexadecane. The use of the nonwoven fabric increased the lipase yield by 130%. Further supplementation of 0.1% (v/v) olive oil to the medium could markedly shorten the fermentation time, and thus increase the volumetric productivity. The use of the nonwoven fabric offered two additional advantages in the lipase fermentation: ease of foam control and favorable partition of lipase in the aqueous phase.
机译：通过不动杆菌属抗辐射的脂肪酶生产是在配备有非织造织物的2-L罐式发酵罐中进行的，该非织造织物由尼龙6纤维制成并涂有疏水性丙烯酸树脂。发酵培养基包含2％（v / v）的正十六烷作为碳源。非织造布的用途旨在分散烃。因此，无需使用乳化剂就可以增加细胞吸收正十六烷的接触表面。当细胞在正十六烷上生长时，发现脂肪酶的形成与生长相关。无纺布的使用使脂肪酶的产率提高了130％。向培养基中进一步添加0.1％（v / v）橄榄油可以显着缩短发酵时间，从而提高容积生产率。使用非织造织物在脂肪酶发酵中提供了两个额外的优点：易于控制泡沫和脂肪酶在水相中的有利分配。

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