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首页> 外文期刊>Biotechnology Progress >Reversible Immobilization of Glucoamylase by Ionic Adsorption on Sepabeads Coated with Polyethyleneimine
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Reversible Immobilization of Glucoamylase by Ionic Adsorption on Sepabeads Coated with Polyethyleneimine

机译：离子吸附在聚乙烯亚胺包覆的Sepabeads上可逆固定糖化淀粉酶

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摘要

Glucoamylase (GA)from Aspergillus niger was immobilized via ionic adsorption onto DEAE-agarose,QIA-Sepabeads,and Sepabeads EC-EP3 supports coated with poly-ethyleneimine (PEI).After optimization of the immobilization conditions (pH,polymer size),it was observed that the adsorption strength was much higher in PEI-Sepabeads than in QIA-Sepabeads or DEAE-supports,requiring very high ionic strength to remove glucoamylase from the PEI-supports (e.g.,1 M NaCl at pH 5.5).Thermal stability and optimal temperature was marginally improved by this immobilization.Recovered activity depended on the substrate used,maltose or starch,except when very low loading was used.The optimization of the loading allowed the preparation of derivatives with 750 lU/g in the hydrolysis of starch,preserving a high percentage of immobilized activity (around 50%).
机译：通过离子吸附将黑曲霉的葡糖淀粉酶（GA）固定在DEAE-琼脂糖，QIA-Sepabeads和Sepabeads EC-EP3载体上，该载体涂覆有聚乙烯亚胺（PEI）。在优化固定条件（pH，聚合物大小）后，将其固定观察到，PEI-Sepabeads的吸附强度比QIA-Sepabeads或DEAE-载体要高得多，需要非常高的离子强度才能从PEI-Sepabeads去除葡糖淀粉酶（例如pH 5.5的1 M NaCl）。固定化可略微提高最佳温度。恢复的活性取决于所用的底物，麦芽糖或淀粉，除非使用非常低的负载量。负载量的优化允许在淀粉水解中制备750 lU / g的衍生物，保留较高百分比的固定化活动（大约50％）。

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