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Extractive Cultivation of Xylanase by Penicillium janthinellum in a Poly(ethylene glycol)/Cashew-Nut Tree Gum Aqueous Two-Phase System

机译:聚乙二醇/腰果树胶水两相体系中花青霉提取木聚糖酶的研究

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Cultivation of the fungus Penicillium janthinellum for xylanase production was studied in a poly(ethylene glycol)/cashew-nut tree gum aqueous two-phase system,using a two-level fractional factorial design.The parameters studied were initial pH,cultivation time,type of agro-industrial residue(oat husk or corn cob),agitation,temperature,and phase-forming polymers.The xylanase produced during fermentation partitioned into the top phase.The agitation and temperature(negative),cultivation time and initial pH(positive)effects proved statistically significant for xylanase production.The highest percentage yield of the xylanase in the top and its production in the top phase,about 97% and 160.7 U/mL,were obtained in cultures of 120 h,40 rpm,25 °C,and pH 5.0.
机译:在聚乙二醇/腰果树胶水两相体系中,采用二级分级因子设计,研究了木霉青霉菌生产木聚糖酶的性能。研究的参数为初始pH,培养时间,类型农业工业残留物(燕麦壳或玉米芯),搅拌,温度和相形成聚合物。发酵过程中产生的木聚糖酶分配到顶层。搅拌和温度(负),培养时间和初始pH(正)结果表明,木聚糖酶的生产具有统计学意义。在120 h,40 rpm,25°C的培养条件下,最高的木聚糖酶收率和最高的木糖酶产量均达到最高,约为97%和160.7 U / mL。和pH 5.0。

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