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首页> 外文期刊>Biotechnology Progress >Effect of Process Variables on Supercritical Fluid Disruption of Ralstonia eutropha Cells for Poly(R-hydroxybutyrate)Recovery
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Effect of Process Variables on Supercritical Fluid Disruption of Ralstonia eutropha Cells for Poly(R-hydroxybutyrate)Recovery

机译：过程变量对富营养的Ralstonia eutropha细胞破坏的影响（R-羟基丁酸酯）

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摘要

This research focuses on the disruption of the Gram-negative bacterium Ralstonia eutropha cells by supercritical CO_2 for polyCR-hydroxybutyrate)(PHB)recovery.The variables affecting cell disruption such as drying strategy,type of modifier,and cultivation time,as well as operating pressure,temperature,and repeated release of supercritical CO_2 pressure,have been studied.Effect of this disruption technique on PHB molecular mass was also investigated.PHB recovery was examined using a combination of this method and chemical pretreatments.For salt pretreatment,the cells were exposed to 140 mM NaCl and heat(60 deg C,1 h).The cells were also exposed to 0.2-0.8%(w/w)NaOH to examine the effect of alkaline pretreatment.Bacterial cells treated in growth phase exhibited less resistance to disruption than nutrient-limited cells in the stationary phase.It was also found that the wet cells could be utilized to recover PHB,but purity of the product was lower than that obtained from freeze-dried cells.Pretreatment with a minimum of 0.4%(w/w)NaOH was necessary to enable complete disruption with two times pressure release.Salt pretreatment was less effective;however,disruption was improved by the application of alkaline shock.The proposed method is economic and comparable with other recovery methods in terms of the percentage of PHB recovery and energy consumption,while it is environmentally more benign.
机译：这项研究的重点是超临界CO_2对革兰氏阴性细菌富营养小球藻（Ralstonia eutropha）细胞的破坏，以回收聚CR-羟基丁酸酯（PHB）。影响细胞分裂的变量如干燥策略，修饰剂类型，培养时间以及操作研究了压力，温度和超临界CO_2压力的反复释放。还研究了这种破坏技术对PHB分子量的影响。结合此方法和化学预处理方法检查了PHB的回收率。暴露于140 mM NaCl中并加热（60摄氏度，1小时）。细胞也暴露于0.2-0.8％（w / w）NaOH中以检查碱性预处理的效果。在生长期中处理的细菌细胞对细菌的抵抗力较小还发现，湿细胞可用于回收PHB，但产物纯度低于冷冻干燥细胞。为了使两次压力释放都能完全破裂，必须使用至少0.4％（w / w）的NaOH进行预处理。盐的预处理效果不佳;但是通过施加碱冲击可以改善破裂效果。在PHB回收率和能源消耗方面，可与其他回收方法相媲美，同时在环境方面更为温和。

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