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首页> 外文期刊>Biotechnology Progress >Optimizing iron supplement strategies for enhanced surfactin production with Bacillus subtilis
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Optimizing iron supplement strategies for enhanced surfactin production with Bacillus subtilis

机译：优化铁补充策略以提高枯草芽孢杆菌的表面活性素生产

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摘要

Supplement of Fe2+ into fermentation medium was utilized as a tool to optimize the iron-mediated enhancement of surfactin production from Bacillus subtilis ATCC 21332. Up to 3000 mg L-1 of surfactin was produced using an iron-enriched minimal salt (MS) medium amended with an optimal Fe2+ dosage of 4.0 mM, leading to 8-fold and 10-fold increase in cell concentration and surfactin yield, respectively, as compared to those without Fe2+ supplement. In addition to resulting in an optimal production yield of surfactin, a supplement of 4.0 mM of Fe2+ also propelled maximum overall surfactin production rate to a highest value of 24 mg L-1 h(-1). Our results also show that production of surfactin followed a growth-associated kinetic model. The best yield coefficient estimated from the model was ca. 162 mg surfactin (g dry cell)(-1). The supernatant of the iron-enriched culture of B. subtilis ATCC 21332 exhibited the ability to emulsify kerosene and achieved a maximum emulsion index (E-24) of 80% for culture supplemented with 4.0 mM of Fe2+. Comparison of emulsion index and the corresponding surfactin production indicates that the emulsification activity was essentially contributed by surfactin.
机译：利用发酵培养基中补充的Fe2 +作为优化铁介导的枯草芽孢杆菌ATCC 21332产生表面活性素的工具。使用改良的富铁最低盐（MS）培养基可生产多达3000 mg L-1的表面活性素。 Fe2 +的最佳剂量为4.0 mM，与不含Fe2 +的那些相比，分别导致细胞浓度和表面活性素产量增加8倍和10倍。除了获得最佳的表面活性素产量外，补充4.0 mM的Fe2 +还可以将最大的表面活性素总生产率提高到24 mg L-1 h（-1）的最高值。我们的结果还表明，表面活性素的产生遵循与生长相关的动力学模型。从模型估计的最佳产量系数为。 162 mg表面活性素（g干细胞）（-1）。枯草芽孢杆菌ATCC 21332的富铁培养物的上清液具有乳化煤油的能力，对于添加了4.0 mM Fe2 +的培养物，其最大乳化指数（E-24）为80％。乳液指数和相应的表面活性素生产的比较表明，乳化活性主要由表面活性素贡献。

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