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Rapid Detection of Pathogenic Bacteria from Fresh Produce by Filtration and Surface-Enhanced Raman Spectroscopy

机译：过滤和表面增强拉曼光谱法快速检测新鲜农产品的致病细菌

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摘要

The detection of Salmonella Poona from cantaloupe cubes and E. coli O157:H7 from lettuce has been explored by using a filtration method and surface-enhanced Raman spectroscopy (SERS) based on vancomycin-functionalized silver nanorod array substrates. It is found that with a two-step filtration process, the limit of detection (LOD) of Salmonella Poona from cantaloupe cubes can be as low as 100 CFU/mL in less than 4 h, whereas the chlorophyll in the lettuce causes severe SERS spectral interference. To improve the LOD of lettuce, a three-step filtration method with a hydrophobic filter is proposed. The hydrophobic filter can effectively eliminate the interferences from chlorophyll and achieve a LOD of 1000 CFU/mL detection of E. coli O157:H7 from lettuce samples within 5 h. With the low LODs and rapid detection time, the SERS biosensing platform has demonstrated its potential as a rapid, simple, and inexpensive means for pathogenic bacteria detection from fresh produce.
机译：通过使用基于万古霉素官能化的银纳米棒阵列基板的过滤方法和表面增强的拉曼光谱（SERS）探索来自甘蔗素立方体和大肠杆菌O157：H7的Salmonella Poona：H7。 结果发现，通过两步过滤过程，来自甘蔗立方体的沙门氏菌的检测（LOD）的极限可以低至100个CFU / ml，而莴苣中的叶绿素会导致严重的SERS光谱 干涉。 为了改善莴苣的床位，提出了一种具有疏水过滤器的三步过滤方法。 疏水过滤器可以有效地消除叶绿素的干扰，并在5小时内从莴苣样品中获得1000 CFU / ml检测的1000cfu / ml检测。 通过低床单和快速检测时间，SERS生物体传感平台已经证明其潜力作为一种快速，简单，廉价的手段，用于从新鲜农产品中检测到致病细菌。

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  • 来源
    《JOM》 |2016年第4期|共7页
  • 作者

    Wu Xiaomeng; Han Caiqin; Chen Jing; Huang Yao-Wen; Zhao Yiping;

  • 作者单位

    Univ Georgia Nanoscale Sci &

    Engn Ctr 220 Riverbend Rd Athens GA 30605 USA;

    Univ Georgia Nanoscale Sci &

    Engn Ctr 220 Riverbend Rd Athens GA 30605 USA;

    Univ Georgia Nanoscale Sci &

    Engn Ctr 220 Riverbend Rd Athens GA 30605 USA;

    Univ Georgia Nanoscale Sci &

    Engn Ctr 220 Riverbend Rd Athens GA 30605 USA;

    Univ Georgia Nanoscale Sci &

    Engn Ctr 220 Riverbend Rd Athens GA 30605 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 有色金属冶炼;金属学与金属工艺;
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