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首页> 外文期刊>Biotechnology Progress >Effect of Various Freezing Parameters on the Immediate Post-Thaw Membrane Integrity of Adipose Tissue Derived Adult Stem Cells
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Effect of Various Freezing Parameters on the Immediate Post-Thaw Membrane Integrity of Adipose Tissue Derived Adult Stem Cells

机译：各种冷冻参数对脂肪组织成体干细胞立即融化后膜完整性的影响

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The effect of four thermal parameters on post-thaw membrane integrity of adipose tissue derived adult stem (ADAS) cells after controlled-rate freezing was investigated with the help of a two-level four-parameter (2~4) experimental design.The four thermal parameters studied were cooling rate (CR),end temperature (ET),hold time (HT),and thawing rate (TR).Several passages,including Passage-0-(PO),Passage-1 (P1),Passage-2 (P2),Passage-3 (P3),and Passage-4 (P4),obtained from the suspended culture of stromal vascular fraction (SVF) of the ADAS cells were used for this study.The two levels (low and high) of the four parameters [CR (1 and 40 deg C/min);ET (-80 and -20 deg C);HT (1 and 15 min);and TR (10 and 200 deg C/min)] are chosen in such a way that they enclosed all parameter values possible using commercially available controlled-rate freezing equipment.Individual effect of each parameter on the immediate post-thaw membrane integrity was determined through the calculation of parameter effect values (E),and any synergy among the parameters on post-thaw membrane integrity was assessed through the calculation of two or more parameter interaction effect values (I).Nonlinearity in the experimental results was represented through the calculation of curvature value (CV).The results suggest that for 99% confidence level the parameters CR and ET have considerable effect on post-thaw membrane integrity of all passages of ADAS cells.A significant individual effect of TR was observed with P3 and P4 cells and a significant two-parameter interaction was observed between CR-ET for all passages.These observed results will be used as a basis to further develop freezing storage protocols of ADAS cells.
机译：借助二级四参数（2〜4）实验设计，研究了四个热参数对脂肪组织来源的成年干细胞（ADAS）解冻后膜完整性的影响。研究的热参数为冷却速率（CR），结束温度（ET），保持时间（HT）和解冻速率（TR）。几道，包括通道0-（PO），通道1（P1），通道-本研究使用从ADAS细胞的基质血管部分（SVF）悬浮培养物中获得的2（P2），Passage-3（P3）和Passage-4（P4）。这两个水平（低和高）在以下四个参数中选择[CR（1和40摄氏度/分钟）; ET（-80和-20摄氏度）; HT（1和15分钟）; TR（10和200摄氏度/分钟）]通过使用参数eff的计算确定每个参数对即时解冻后膜完整性的影响值（E），以及通过计算两个或多个参数相互作用效应值（I）评估参数之间对融化后膜完整性的任何协同作用。通过计算曲率值（CV）表示实验结果中的非线性）。结果表明，对于99％的置信度，参数CR和ET对ADAS细胞所有传代的解冻后膜完整性都有相当大的影响.P3和P4细胞对TR的影响显着，而两个在所有传代中都观察到CR-ET之间的参数交互作用。这些观察到的结果将用作进一步制定ADAS细胞冷冻保存方案的基础。

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