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首页> 外文期刊>Journal of Analytical Toxicology >Application of High-Resolution UPLC-MSE/TOF Confirmation in Forensic Urine Drug Screening by UPLC-MS/MS
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Application of High-Resolution UPLC-MSE/TOF Confirmation in Forensic Urine Drug Screening by UPLC-MS/MS

机译：高分辨率UPLC-MSE / TOF确认在UPLC-MS / MS中的尿液药物筛选中的应用

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摘要

The transition from presumptive (immunoassay) drug screening to definitive screening has continued in the practice of analytical toxicology. Development of a ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) screening method for over sixty drugs and metabolites (analytes) in urine has been reported by the authors and has been applied in probation, drug court, social services, chemical dependency, pain management and addiction medicine casework. Testing by the definitive screening method has increased both the rate and diversity of initial-positive drug findings, due to the lower positive thresholds and wider panel of analytes. Use of definitive screening in forensic casework, however, requires retesting of initial-positive analytes using a second method based upon a different analytical technique with at least equivalent sensitivity and selectivity. Consequently, a UPLC-MSE/TOF method for confirmation of the initial-positive analytes has been adapted; the method is for targeted confirmation and is based upon an alternate mass spectrometry technology and column separation. Both the initial screen and the confirmatory analysis employ threshold accurate calibration for normalization of matrix effects, without the use of stable isotopes. Validation and application of the complete workflow, in forensic urine drug testing casework, is reported.
机译：在分析毒理学的实践中，从推定（免疫测定）药物筛查的过渡到明确的筛查仍在继续。作者据报道，尿液中超过60种药物和代谢物（分析物）的超高效液相色谱 - 串联质谱（UPLC-MS / MS）筛选方法，并已申请试用，药物法院，社会服务，化学依赖，疼痛管理和成瘾医学案例。由于较低的正阈值和更广泛的分析板，通过最终筛选方法测试增加了初始阳性药物发现的速率和多样性。然而，在法医案例中使用最终筛选需要使用基于不同的分析技术的第二种方法在具有至少等效灵敏度和选择性的不同分析技术的情况下重新测试初始阳性分析物。因此，用于确认初始阳性分析物的UPLC-MSE / TOF方法已经调整;该方法用于靶向确认，基于交替的质谱技术和柱分离。初始屏幕和确认分析都采用阈值精确校准进行常规的基质效应，而不使用稳定同位素。报道了验证和应用完整的工作流程，在法医尿药物测试案例中。

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