...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Animal Physiology and Animal Nutrition >The influence of a simulated digest of an equine dietary feed additive G's formula on contractile activity of gastric smooth muscle in vitro
【24h】

The influence of a simulated digest of an equine dietary feed additive G's formula on contractile activity of gastric smooth muscle in vitro

机译：马膳食饲料添加剂G的模拟消化对体外胃平滑肌收缩活性的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

G's Formula is a novel equine feed additive formulated to promote optimal GI function. The objective of this study was to determine whether the addition of a simulated digest of the composite feed additive G's Formula (FA) would alter the contractile response of gastric smooth muscle to acetylcholine (Ach). Smooth muscle strips from porcine stomachs were excised and attached to an isometric force transducer. An experiment was run to compare tissue contraction between tissue exposed to FA (FA; n = 8, simulated digest of FA was added to the bath) and control tissue (CO; n = 8, no additions made). Increasing concentrations of Ach were added into the bath such that the concentration increased from 10(-8)-10(-3) M. Based on the analysis of these data, a difference between FA and CO was observed. Therefore, another trial was run which included a blank group (BL n = 6) in which the tissue was exposed to the simulated digest without FA. More CO (n = 5) and FA (n = 4) tissue was also run. Force was compared to baseline and between groups. In FA group, mean force for 1-min following all Ach additions was higher than baseline (p < .05) and by 2-min the integral-under-force/time curve (AUC) was higher than baseline from 10(-7)-10(-3) M compared to lower concentrations of Ach in both CO (10(-6) M for both) and BL (10(-5) M and 10(-6) M, respectively). By 8-min AUC of all Ach concentrations were higher than baseline in FA compared to an Ach of 10(-6) M in both CO and BL. A simulated digest of FA appears to sensitize gastric smooth muscle to Ach in vitro. FA may increase GI contractility, and the functional effect of this should be studied further in vivo.
机译：G的公式是一种新型的标牌饲料添加剂，用于促进最佳GI功能。本研究的目的是确定复合饲料添加剂G's公式（FA）的模拟摘要是否会改变胃平滑肌对乙酰胆碱（ACH）的收缩响应。切除猪胃的平滑肌条被切除并连接到等距力传感器。运行实验以比较暴露于Fa（Fa; n = 8的组织之间的组织收缩，将Fa的模拟摘要添加到浴中）和控制组织（CO; n = 8，没有添加）。将浓度的ACH增加到浴中，使得浓度从10（-8）-10（-3）M增加。基于这些数据的分析，观察到FA和CO之间的差异。因此，运行另一种试验，其包括空白组（BL n = 6），其中组织暴露于没有FA的模拟摘要。还运行更多CO（n = 5）和FA（n = 4）组织。将力与基线和组之间进行比较。在FA组中，在所有ACH添加后1分钟的平均力高于基线（P <.05）和2分钟，整体欠力/时间曲线（AUC）高于基线（-7） ）-10（-3）M与诸如两者（10（-6）m的较低浓度的ACH相比）和BL（10（-5）m和10（-6）m）。在CO和BL中的10（-6）m的ACH相比，所有ACH浓度的8分钟的AUC均高于FA的基线。 FA的模拟摘要似乎将胃平滑肌敏化到体外患者。 FA可能会增加GI收缩性，并且应在体内进一步研究这种功能效果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号