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首页> 外文期刊>Journal of Animal Physiology and Animal Nutrition >Dietary guanidinoacetic acid improves the growth performance and skeletal muscle development of finishing pigs through changing myogenic gene expression and myofibre characteristics
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Dietary guanidinoacetic acid improves the growth performance and skeletal muscle development of finishing pigs through changing myogenic gene expression and myofibre characteristics

机译:通过改变肌遗传基因表达和肌纤维特征,提高膳食胍基乙酸的生长性能和骨骼肌开发

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摘要

This study aimed to evaluate the effects of dietary guanidine acetic acid (GAA) supplementation on growth performance, carcass traits and the expression of muscle growth-related genes in finishing pigs. A total of 128 (81.03 +/- 1.09 kg body weight) crossbred pigs (Duroc x Landrace xYorkshire) were blocked by body weight and allotted to 16 pens (eight pigs per pen), and pens were randomly assigned within blocks to one of five dietary treatments, with a basal diet (control group) or a basal diet supplemented with 0.03%, 0.06% and 0.09% GAA respectively. During the 60-day trial, GAA increased the average dairy gain (ADG) and average daily feed intake (ADFI) (p < .05). The back fat thickness of pigs fed 0.06% GAA was lower than other groups (p < .05). Pigs fed 0.06% GAA had improved lean meat percentage, loin muscle area, shear force and cross-sectional area of muscle fibre in comparison with control group (p < .05). The drop loss and the muscle fibre density in pigs fed 0.06% GAA were lower than control (p < .05). In addition, dietary GAA enhanced the expression of myosin heavy chain gene (MYH4), myogenic determination (Myod) and myogenic factor 5 (Myf5) in longissimus dorsi and carnitine palmitoyltransferase-1(CPT-1) in liver (p < .05). Meanwhile, GAA decreased the expression of Myostatin in longissimus dorsi and fatty acid synthase (FAS) in liver (p < .05). In conclusion, our results showed that appropriate dietary GAA supplementation (0.06%) promotes skeletal muscle development through changing myogenic gene expression and myofibre characteristics.
机译:本研究旨在评估膳食胍醋酸(Gaa)对猪肉脂肪术,胴体性状和肌肉生长相关基因表达的影响。总共128名(81.03 +/- 1.09公斤体重)杂交猪(Duroc x Landrace Xyorkshire)被体重阻挡并分配到16张钢笔(每支笔八猪),并且在块中随机分配钢笔到五个中饮食治疗,基础饮食(对照组)或含有0.03%,0.06%和0.09%Gaa的基础饮食。在60天的试验中,GAA增加了平均乳制品增益(ADG)和平均每日饲料摄入量(ADFI)(P <.05)。喂食0.06%Gaa的猪的背脂肪厚度低于其他基团(P <.05)。饲喂0.06%Gaa的猪具有改善的瘦肉百分比,腰部肌肉区域,肌纤维的剪切力和横截面积,与对照组相比(P <.05)。饲料0.06%Gaa的猪的损失和肌纤维密度低于对照(P <.05)。此外,膳食Gaa在Longissimus Dorsi和肉氨基棕榈酰转移酶-1(CPT-1)中增强了肌球蛋白重链基因(MYH4),肌原测定(MYF5)和肌原素因子5(MYF5)的表达(P <.05) 。同时,Gaa在肝脏中的长生物Dorsi和脂肪酸合成酶(Fas)中的肌球蛋白的表达(P <.05)。总之,我们的结果表明,通过改变肌遗传基因表达和肌纤维特征,促进骨骼肌发育的适当饮食Gaa补充(0.06%)。

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