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首页> 外文期刊>Journal of Animal Physiology and Animal Nutrition >Effect of cereal straw and alfalfa hay diet on amino acid profile of gastrointestinal digesta in lactating dairy cows
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Effect of cereal straw and alfalfa hay diet on amino acid profile of gastrointestinal digesta in lactating dairy cows

机译：谷物秸秆与苜蓿干草饮食对泌乳奶牛胃肠内切口氨基酸谱的影响

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摘要

This study was conducted to evaluate the effects of replacing alfalfa hay with rice straw (RS) or corn stover (CS) on amino acid (AA) profiles of gastrointestinal digesta in lactating cows. Eighteen lactating dairy cows were randomly assigned to one of three groups (n=6) and fed identical concentrate and corn silage with different forages on dry matter basis: (i) 23% alfalfa hay and 7% Chinese wild rye hay (AH); (ii) 30% CS; and (iii) 30% RS. After the 14-week feeding, a total of 18 cows were slaughtered to collect digesta from four representative organs, including rumen, omasum, duodenum and jejunum. The AA profiles of ruminal microbial fraction were similar among the treatments, except for greater Arg in cows fed RS than in cows fed AH or CS. Most of the analysed AA changed under different diets. Significant differences were found among the microbial fraction, rumen fluid and rumen digesta, with greater essential AA in digesta than in microbial fraction or rumen fluid and greater essential AA in microbial fraction than rumen fluid. Significant differences in individual AA profiles of digesta and relevant fluid were found across the four representative digestive tract parts, including rumen, omasum, duodenum and jejunum, showing much lower Leu proportion in CS and RS than in AH in duodenal fluid. In summary, ruminal microbes may prefer using essential AA, rather than non-essential AA. The AA profile of ruminal microbes was constant except for Arg. The AA composition of digesta across the four digestive tracts changed dramatically, which indicated differences in the ability and efficiency of AA absorption. The lower duodenum absorbable Leu proportion in cows fed CS or RS indicated the shortage of Leu in CS or RS diets, which might also restrict the balanced AA absorption.
机译：进行了本研究以评估用稻草（RS）或玉米秸秆（CS）在泌乳奶牛中胃肠道消化物的氨基酸（AA）型胶质秸秆（CS）替代苜蓿干草的影响。将十八乳奶牛随机分配给三组（n = 6）中的一种（N = 6），并在干物质上用不同的饲料喂食相同的浓缩物和玉米青贮：（i）23％苜蓿干草和7％中国野生黑麦干草（啊）; （ii）30％CS; （iii）30％卢比。 14周喂养后，共屠宰了18牛，以从四个代表性器官收集Digesta，包括瘤胃，Omasum，Duodenum和Jejunum。在治疗中，瘤胃微生物分数的AA型材在喂养牛喂养rs的牛群中的大arg之外，除了喂养的奶牛αh或cs。大多数分析的AA在不同的饮食下发生了变化。在微生物馏分，瘤胃液和瘤胃馏分中发现显着差异，在Digesta中具有更大的必需AA，而不是在微生物馏分中的微生物馏分或瘤胃流体中的更大基本AA，而不是瘤胃流体。在四个代表消化道零下，包括瘤胃，omaasum，十二指肠和jejunum，在四个代表性消化道部分中发现了Digesta和相关流体的个体差异的显着差异。总之，瘤胃微生物可以使用必需的AA，而不是非必要的AA。除arg外，瘤胃微生物的AA轮廓是恒定的。跨越四种消化道的Digesta的AA组成显着变化，这表明AA吸收能力和效率的差异。奶牛中的较低的十二指肠可吸收血清比例CS或Rs表示CS或RS饮食中Leu的短缺，这也可能限制平衡的AA吸收。

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