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首页> 外文期刊>Journal of Animal Physiology and Animal Nutrition >Alterations in fermentation parameters during and after induction of a subacute rumen acidosis in the rumen simulation technique
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Alterations in fermentation parameters during and after induction of a subacute rumen acidosis in the rumen simulation technique

机译：瘤胃模拟技术中诱导亚急性瘤胃酸中毒期间和后的发酵参数的改变

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摘要

Subacute rumen acidosis (SARA) is a common problem in dairy cattle. High-concentrate rations lead to an accumulation of short-chain fatty acids (SCFA) in the rumen and a subsequent decrease in ruminal pH. As SARA impairs animal welfare and productivity, numerous in vivo studies are focusing on evaluation of prevention strategies. In vitro models can support this research and reduce animal numbers and experimental costs. We used different diets and buffer compositions to induce SARA in the rumen simulation technique (Rusitec) and investigated the recovery process. The experiment consisted of an equilibration period (7 days), a first control period, a SARA period and a second control period (5 days each). During the SARA period, SARA was induced by infusing SARA1 or SARA2 buffer with reduced bicarbonate (20 mmol/L and 25 mmol/L) and phosphate (both 10 mmol/L) contents compared to a modified McDougall's buffer (bicarbonate 97.9 mmol/L, phosphates 20 mmol/L). Additionally, we compared three feeding strategies, which differed in the concentrate-to-roughage ratio (30:70, 70:30, changing ratio: 30% concentrate in control periods and 70% concentrate in SARA period). During the SARA period, the pH decreased to a constant value below the SARA thresholds of pH 5.8 and 5.6, whereas lactate concentrations remained low. The total SCFA production rate declined 3 days after SARA induction, and the molar proportion of acetate decreased. The decrease in pH and SCFA production was more pronounced for SARA1 buffer. The high-concentrate diet reduced the molar proportion of acetate and increased NH3-N concentrations. During the second control period, most parameters recovered. In conclusion, SARA conditions were successfully induced in the Rusitec. However, we observed a higher influence of buffer composition than of concentrate proportions on most biochemical parameters. Nearly all changes were reversible. This model can be applied to test acidosis prevention strategies prior to animal experiments.
机译：亚急性瘤胃酸中毒（Sara）是奶牛的常见问题。高浓缩口粮导致瘤胃中短链脂肪酸（SCFA）积聚，随后的瘤胃pH降低。随着萨拉损害动物福利和生产力，众多体内研究专注于评估预防策略。体外模型可以支持这项研究并降低动物数量和实验成本。我们使用不同的饮食和缓冲组合物在瘤胃模拟技术（Rusitec）中诱导Sara并调查恢复过程。该实验由平衡周期（7天），第一控制期，SARA周期和第二个控制期（每个5天）组成。在SARA期间，通过将SARA1或SARA2缓冲液输注碳酸氢盐（20mmol / L和25mmol / L）和与改性的McDougall的缓冲液（碳酸氢盐97.9mmol / L相比，通过减少碳酸氢盐（20mmol / L和25mmol / L）和磷酸盐（10mmol / L）含量来诱导Sara。 ，磷酸盐20mmol / l）。此外，我们比较了三种饲养策略，其浓缩浓缩率与粗饲料率不同（30:70,70:30，变化的比例：30％的浓缩物在控制期中，在Sara时期的70％浓缩物）。在SARA期间，pH降低至低于PH 5.8和5.6的SARA阈值的恒定值，而乳酸浓度仍然低。 SARA诱导后，SCFA总生产率总数下降，醋酸盐的摩尔比例降低。对于Sara1缓冲液，pH和SCFA产生的降低更为显着。高浓缩饮食降低了乙酸盐的摩尔比例和增加的NH 3-N浓度。在第二个控制期间，大多数参数恢复。总之，萨拉病症已成功诱导在荷斯尼特中。然而，我们观察到缓冲剂组合物的较高影响小于大多数生化参数的浓缩比例。几乎所有变化都是可逆的。该模型可用于在动物实验前测试酸中毒预防策略。

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