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首页> 外文期刊>Journal of AOAC International >Development and In-House Validation of an LC-MS and LC-MS/MS Assay for the Determination of Food Fraud for Different Fish Species
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Development and In-House Validation of an LC-MS and LC-MS/MS Assay for the Determination of Food Fraud for Different Fish Species

机译：LC-MS和LC-MS / MS检测的开发和内部验证用于确定不同鱼类的食物欺诈

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Background: Fish and fish products are one of the most important food sources of high commercial interest. The global food trade and the associated risks are constantly presenting new challenges to consumer protection and public authorities, which, among other things, demand state-of-the-art analytical methods to ensure food authenticity. Objective: The establishment of MS-based strategies plays a decisive role alongside the (further) development of ELISA-or DNA-oriented methods. Methods: In the present work, therefore, the development and in-house validation of an LC-MS and LC-MS/MS-based assay for authenticity testing of certain fish species is described. Results: Based on the execution of a validated bottom-up LC-electrospray-MS and MS/MS assay and multivariate analysis, the commercially available species Lutjanus malabaricus (red snapper) and Sebastes spp. (redfish) are distinguished from each other, whereas an additional 68 samples [nine additional marine species such as pangasius (Pangasianodon hypophthalmus), salmon (Salmo salar), turbot (Scophthalmus maximus), plaice (Pleuronectes platessa), sole (Solea solea), lemon sole (Glyptocephalus cynoglossus), halibut (Reinhardtius hypoglossoides), red salmon (Oncorhynchus nerka), and great scallop (Pecten jacobaeus)] served as blinded negative controls to ensure the specificity of the assay. Conclusions and Highlights: A promising LC-MS and LC-MSMS based assay has been developed that could enable the detection of fish fraud at the protein level in the future.
机译：背景：鱼类和鱼类产品是高商业利益最重要的食物来源之一。全球粮食贸易和相关风险不断为消费者保护和公共当局呈现出新的挑战，其中包括最先进的分析方法，以确保食品真实性。目的：建立基于MS的策略，并披露了（进一步）的ELISA或DNA导向方法的果断作用。方法：在本作本作中，描述了LC-MS和LC-MS / MS的基于LC-MS和MS的确定的某些鱼类真实性测试的开发和内部验证。结果：基于验证的自下而上LC-Electrospray-MS和MS / MS测定和多变量分析的执行，市售物种Lutjanus Malabaricus（红鲷鱼）和Sebastes SPP。 （Redfish）彼此区分，而另外68个样本[九个额外的海洋物种，如Pangasius（Pangasianodon次疗法），三文鱼（Salmo Salar），Turbot（Scophthalmus Maximus），Plaice（Pleuronectes platessa），唯一（solea solea） ，柠檬鞋底（Glyptocephalus cynoglossus），大纤维（Reinhardtius hypovlossoides），红鲑鱼（牛孢子素Nerka）和大扇贝（Pecten Jacobaeus）]用作盲的阴性对照，以确保测定的特异性。结论和亮点：已经开发了一个有前途的LC-MS和基于LC-MSMS的测定，可以在未来检测蛋白质水平的鱼欺诈。

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