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首页> 外文期刊>Journal of AOAC International >Romer Labs RapidChek?Listeria monocytogenes Test System for the Detection of L. monocytogenes on Selected Foods and Environmental Surfaces
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Romer Labs RapidChek?Listeria monocytogenes Test System for the Detection of L. monocytogenes on Selected Foods and Environmental Surfaces

机译：ROMER LABS RAPINCHEK？Histeria单核细胞增生检测选择于选定食品和环境表面的L.单核细胞增生的测试系统

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The Romer Labs RapidChek?Listeria monocytogenes test system (Performance Tested Method 011805) was validated against the U.S. Department of Agriculture-Food Safety and Inspection Service Microbiology Laboratory Guidebook (USDA-FSIS/MLG), U.S. Food and DrugAssociation Bacteriological Analytical Manual (FDA/BAM), and AOAC Official Methods of Analysis (AOAC/OMA) cultural reference methods for the detection of L. monocytogenes on selected foods including hot dogs, frozen cooked breaded chicken, frozen cooked shrimp, cured ham,and ice cream, and environmental surfaces including stainless steel and plastic in an unpaired study design. The RapidChek method uses a proprietary enrichment media system, a 44–48?h enrichment at 30 ± 1°C, and detects L. monocytogenes on an immunochromatographiclateral flow device within 10?min. Different L. monocytogenes strains were used to spike each of the matrixes. Samples were confirmed based on the reference method confirmations and an alternate confirmation method. A total of 140 low-level spiked samples were tested by the RapidChekmethod after enrichment for 44–48?h in parallel with the cultural reference method. There were 88 RapidChek presumptive positives. One of the presumptive positives was not confirmed culturally. Additionally, one of the culturally confirmed samples did not exhibit a presumptive positive.No difference between the alternate confirmation method and reference confirmation method was observed. The respective cultural reference methods (USDA-FSIS/MLG, FDA/BAM, and AOAC/OMA) produced a total of 63 confirmed positive results. Nonspiked samples from all foods were reported as negativefor L. monocytogenes by all methods. Probability of detection analysis demonstrated no significant differences in the number of positive samples detected by the RapidChek method and the respective cultural reference method.
机译：ROMER LABS RAPINCHEK？Histeria单核细胞膜测试系统（性能测试方法011805）对美国农业部 - 食品安全和检验服务微生物实验室指南（USDA-FSIS / MLG），美国食品和药物分析细菌学分析手册（FDA / BAM）和AOAC官方分析方法（AOAC / OMA）文化参考方法，用于检测L.单核细胞增生的选定食品，包括热狗，冷冻煮熟的鸡肉，冷冻煮熟的虾，腌制火腿和冰淇淋，以及环境表面包括不配对的研究设计中的不锈钢和塑料。 RapidChek方法使用专有的富集介质系统，44＆amp;＃8211; 48？H富集在30±1℃，并在10℃内检测免疫色谱分流装置上的L.单核细胞增生。不同的L.单核细胞元菌株用于刺激每个基质。基于参考方法确认和交替确认方法确认样品。在富集44＆amp的富集后，通过RapidChekmethod测试了总共140个低水平的样品;与文化参考方法平行，48·H平行。有88个rapychek推定阳性。其中一个推定积极因素没有在文化上证实。另外，其中一种培养证实的样品未表现出推测阳性。观察到的替代确认方法与参考确认方法之间的差异。相应的文化参考方法（USDA-FSIS / MLG，FDA / BAM和AOAC / OMA）共产生63例确诊的阳性结果。所有食物的非膨胀样品被据报道，所有方法都是单核细胞元的负面。检测分析的概率表明，RapidChek方法检测的阳性样本数量和各自的文化参考方法没有显着差异。

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