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首页> 外文期刊>Journal of applied toxicology >Electronic cigarette liquid exposure induces flavor‐dependent osteotoxicity and increases expression of a key bone marker, collagen type I
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Electronic cigarette liquid exposure induces flavor‐dependent osteotoxicity and increases expression of a key bone marker, collagen type I

机译：电子烟液体暴露会诱导风味依赖性骨毒性，并增加关键骨标记物的表达，胶原蛋白I型

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Abstract Electronic cigarettes (ecigarettes) are nicotine delivery devices advertised as a healthier alternative to conventional tobacco products, but their rapid rise in popularity outpaces research on potential health consequences. As conventional tobacco use is a risk factor for osteoporosis, this study examines whether exposure to electronic liquid (eliquid) used in ecigarettes affects boneforming osteoblasts. Human MG63 and Saos2 osteoblastlike cells were treated for 48爃ours with 0.004%4.0% dilutions of commercially available eliquids of various flavors with or without nicotine. Changes in cell viability and key osteoblast markers, runtrelated transcription factor 2 and Col1a1, were assessed. With all eliquids tested, cell viability decreased in a dosedependent manner, which was least pronounced in flavorless eliquids, most pronounced in cinnamonflavored eliquids and occurred independently of nicotine. Col1a1, but not runtrelated transcription factor 2, mRNA expression was upregulated in response to coffeeflavored and fruitflavored eliquids. Cells treated with a noncytotoxic concentration of fruitflavored Mango Blast eliquid with or without nicotine showed significantly increased collagen type I protein expression compared to culture medium only. We conclude that the degree of osteotoxicity is flavordependent and occurs independently of nicotine and that flavored eliquids reveal collagen type I as a potential target in osteoblasts. This study elucidates potential consequences of ecigarette use in bone.
机译：摘要电子香烟（ecigarettes）是尼古丁交付设备，作为传统烟草产品的更健康的替代品，但他们对潜在健康后果的迅速崛起。随着常规烟草的使用是骨质疏松症的危险因素，该研究检查了ecigarettes中使用的电子液体（灰度）是否会影响骨形成的成骨细胞。将人Mg63和Saos2骨缩醛纤维细胞处理48℃，其各种香料的0.004％4.0％稀释液，有或没有尼古丁。评估细胞活力和键骨细胞标记，循环转录因子2和COL1A1的变化。通过测试的所有灰分，细胞活力以依赖性的方式降低，其在无味的菌液中最不明显，最显着的肉核风格的灰醇，并且独立于尼古丁而发生。 COL1A1，但不是循环的转录因子2，响应于CoffeeFlovered和Froupforly的含量，使MRNA表达上调。用或不含尼古丁的含有或不含尼古丁的Frencforavored Mango Blast Eliquid的非胞素毒性浓度处理的细胞显示出与培养基相比显着增加了胶原型I蛋白表达。我们得出结论，骨毒性程度是氟胺依赖性，并且独立地发生尼古丁，并且调味的电量会显示胶原蛋白I型作为成骨细胞中的潜在靶标。本研究阐明了生态素使用骨骼的潜在后果。

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