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首页> 外文期刊>Journal of biochemical and molecular toxicology >Effect of in utero and postnatal exposure to environmental tobacco smoke on the developmental expression of pulmonary cytochrome P450 monooxygenases.
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Effect of in utero and postnatal exposure to environmental tobacco smoke on the developmental expression of pulmonary cytochrome P450 monooxygenases.

机译：子宫内外接触对环境烟草烟雾对肺细胞色素P450单氧基酶发育表达的影响。

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摘要

Pulmonary cytochrome P450 monooxygenases metabolize xenobiotic chemicals, including those found in environmental tobacco smoke (ETS). Exposure to ETS beginning at birth has been shown to induce the P450 CYP1A1 by seven days of life. The effects of perinatal exposure to ETS of the rat lung on the expression of CYP1A1, 1B1, 2B1, and NADPH cytochrome P450 reductase were measured using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Timed pregnant dams and their pups were exposed to aged and diluted sidestream cigarette smoke (ADSS) as a surrogate for ETS for four hours/ day from gestational day 5 through postnatal day 21. For all genes analyzed, mRNA could be detected in the fetal lung beginning at gestational day 17 but were not altered by ADSS. In contrast, intraperitoneal injection of dams with beta-naphthoflavone significantly elevated both CYP1A1 and 1B1 at gestational day 21, indicating that these genes are inducible. Continued exposure to ADSS significantly induced CYP1A1 but not other P450 genes as early as one day after birth.. We conclude that (1) ADSS induces pulmonary CYP1A1 in the first day of life; (2) fetal cytochrome P450 genes are not induced by maternal exposure to ADSS; and (3) in the fetal lung, CYP1A1 and 1B1 can be induced by beta-naphthoflavone.
机译：肺细胞色素P450单氧基酶代谢异黄素化学品，包括在环境烟草烟雾（ETS）中发现的那些。已经显示出在出生时开始的ETS暴露，诱导P450 CYP1A1七天的生命。使用半定量逆转录酶 - 聚合酶链反应（RT-PCR）测定测量CYP1A1,1，1B1,2B1和NADPH细胞色素P450还原酶对大鼠肺的静脉肺的影响。定时怀孕水坝及其幼崽暴露于老年人和稀释的侧流卷烟烟雾（ADSS）作为ETS的替代品，从前一天的第5天从妊娠第5天到妊娠第5天的替代品。对于分析的所有基因，可以在胎儿肺中检测mRNA从妊娠第17天开始，但没有被广告改变。相比之下，在妊娠期21天在妊娠期21天，腹腔内注射β-萘酚的坝体显着升高了Cyp1a1和1b1，表明这些基因是诱导的。继续接触ADSS显着诱导CYP1A1，但在出生后一天，并不是其他P450基因。我们得出结论（1）ADSS在寿命的第一天诱导肺CYP1A1; （2）胎儿细胞色素P450基因未被母体暴露于ADS; （3）在胎儿肺中，CYP1A1和1B1可以通过β-萘氟洛酮诱导。

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