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首页> 外文期刊>Journal of biomaterials applications >In vitro endothelial differentiation evaluation on polycaprolactone-methoxy polyethylene glycol electrospun membrane and fabrication of multilayered small-diameter hybrid vascular graft
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In vitro endothelial differentiation evaluation on polycaprolactone-methoxy polyethylene glycol electrospun membrane and fabrication of multilayered small-diameter hybrid vascular graft

机译：多己内酯 - 甲氧基聚乙二醇电纺膜的体外内皮分化评价及多层小直径杂交血管移植物的制备

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During development of functional tissue engineered vascular graft, ensuring endothelialization at inner layer and mechanically strong outer layer is considered as challenging. The study objective was to fabricate a multilayered artificial blood vessel by dual syringe electrospinning technique. The presented graft was composed of polycaprolactone-methoxy polyethylene glycol which acts as an inner layer whereas Poly [4,4'-methylenebis(phenyl isocyanate)-alt-1,4-butanediol/di(propylene glycol)/polycaprolactone] (PUPCL) (12% w/v) was incorporated as the outer layer. To find out the optimum inner layer, different polycaprolactone-methoxy polyethylene glycol electrospun membranes varying methoxy polyethylene glycol content (0, 5, 10, 20, and 30% w/v) were prepared and evaluated. The characterizations revealed that blending of methoxy polyethylene glycol with polycaprolactone improved hydrophilicity and antithrombogenic properties compared to polycaprolactone or polyurethane polycaprolactone membrane. During in vitro assessment, the optimized polycaprolactone-20% methoxy polyethylene glycol membrane reinforced a greater differentiation of rat bone marrow stem cell into endothelial cells at both mRNA and protein levels by significantly inducing endothelial markers (CD31, Flk-1, and von Willebrand factor) expression. The polyurethane polycaprolactone membrane showed higher mechanical strength than polycaprolactone-methoxy polyethylene glycol while multilayered polycaprolactone-20% methoxy polyethylene glycol/polyurethane polycaprolactone vascular graft exhibited better result compared to single form. Therefore, the strong and bioactive polycaprolactone-20% methoxy polyethylene glycol/polyurethane polycaprolactone small diameter graft (<2 mm) prepared by simple fabrication process could be a promising preliminary platform for vascular tissue engineering application studies.
机译：在开发功能组织工程化血管移植过程中，确保内层内皮和机械强外层被认为是挑战性的。研究目的是通过双注射器静电纺丝技术制造多层人工血管。所提出的移植物由聚己内酯 - 甲氧基聚乙二醇组成，其用作内层，而聚[4,4'-亚甲基（苯基异氰酸酯）-1,4-丁二醇/二（丙二醇）/聚己内酯]（PPCL） （12％w / v）掺入外层。为了找出最佳内层，制备和评价不同的聚己内酯 - 甲氧基聚乙二醇电纺膜（0,5,10,20和30％w / v）。表征显示，与聚己内酯或聚氨酯聚己内酯膜相比，具有聚己内酯的甲氧基聚乙二醇的混合改善了亲水性和抗血栓形成性能。在体外评估期间，优化的聚己内酯-20％甲氧基聚乙二醇膜通过显着诱导内皮标记物（CD31，FLK-1和VON WILEBRAND系数，增强了大鼠骨髓干细胞进入内皮细胞中的大鼠骨髓干细胞进入内皮细胞。 ） 表达。聚氨酯聚己内酯膜与多己内酯 - 甲氧基聚乙二醇相比，聚氨酯聚己内酯膜显示出比多碳酮酮 - 甲氧基聚乙二醇更高的机械强度，而多层聚己内酯-20％甲氧基聚乙二醇/聚氨酯聚丙烯酸酮血管移植物与单一形式相比表现出更好的效果。因此，通过简单的制造过程制备的强和生物活性聚己内酯-20％甲氧基聚乙二醇-20％甲氧基聚乙二醇-20％甲氧基聚乙二醇/聚氨酯聚丙酮（<2mm）可能是血管组织工程应用研究的有希望的初步平台。

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