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首页> 外文期刊>Journal of biomedical materials research, Part A >Influence of different modifications of a calcium phosphate bone cement on adhesion, proliferation, and osteogenic differentiation of human bone marrow stromal cells
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Influence of different modifications of a calcium phosphate bone cement on adhesion, proliferation, and osteogenic differentiation of human bone marrow stromal cells

机译：磷酸钙骨水泥对磷酸钙骨水泥对粘附，增殖和骨质发生分化的影响

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摘要

Collagen and noncollagenous proteins of the extracellular bone matrix are able to stimulate bone cell activities and bone healing. The modification of calcium phosphate bone cements used as temporary bone replacement materials with these proteins seems to be a promising approach to accelerate new bone formation. In this study, we investigated adhesion, proliferation, and osteogenic differentiation of human bone marrow stromal cells (hBMSC) on Biocement D/collagen composites which have been modified with osteocalcin and O-phospho-L-serine. Modification with osteocalcin was carried out by its addition to the cement precursor before setting as well as by functionalization of the cement samples after setting and sterilization. hBMSC were cultured on these samples for 28 days with and without osteogenic supplements. We found a positive impact especially of the phosphoserine-modifications but also of both osteocalcin-modifications on differentiation of hBMSC indicated by higher expression of the osteoblastic markers matrix metalloproteinase-13 and bone sialo protein II. For hBMSC cultured on phosphoserinecontaining composites, an increased proliferation has been observed. However, in case of the osteocalcin-modified samples, only osteocalcin adsorbed after setting and sterilization of the cement samples was able to promote initial adhesion and proliferation of hBMSC. The addition of osteocalcin before setting results in a finer microstructure but the biological activity of osteocalcin might be impaired due to the sterilization process. Thus, our data indicate that the initial adhesion and proliferation of hBMSC is enhanced rather by the biological activity of osteocalcin than by the finer microstructure.
机译：细胞外骨基质的胶原蛋白和非胶原蛋白能够刺激骨细胞活性和骨愈合。用这些蛋白质用作临时骨置换材料的磷酸钙骨水泥的改性似乎是加速新骨形成的有希望的方法。在本研究中，我们研究了用骨钙素和O-磷酸-1-丝氨酸改性的生物骨髓基质细胞（HBMSC）对人骨髓基质细胞（HBMSC）的粘附性，增殖和骨质发生分化。通过在设置和灭菌后的水泥样品的官能化之前，通过添加到水泥前体的补充，通过加入骨钙蛋白进行修饰。 HBMSC在这些样品上培养28天，没有成骨补充剂。我们发现尤其是磷源 - 修饰的积极影响，而且还对骨钙素改性的骨钙素 - 改性，通过骨细胞标志物基质金属蛋白-13和骨唾液蛋白II的较高表达表示的HBMSC分化。对于在磷酸骨复合材料上培养的HBMSC，已经观察到增加的增殖。然而，在骨蛋白改性样品的情况下，在设置和灭菌水泥样品后，只吸附的骨钙素能够促进HBMSC的初始粘附和增殖。在设定之前添加骨钙蛋白在较细微的微观结构中，但由于灭菌方法，骨钙素的生物活性可能会受到损害。因此，我们的数据表明HBMSC的初始粘附性和增殖增强，而不是骨钙素的生物活性而不是通过更细胞结构进行增强。

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