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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Physico‐chemical and in vitro in vitro cellular properties of different calcium phosphate‐bioactive glass composite chitosan‐collagen (CaP@ChiCol) for bone scaffolds
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Physico‐chemical and in vitro in vitro cellular properties of different calcium phosphate‐bioactive glass composite chitosan‐collagen (CaP@ChiCol) for bone scaffolds

机译：不同磷酸钙 - 生物活性玻璃复合壳聚糖 - 胶原蛋白（CAP Zhicol）的物理化学和体外体外细胞性质

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Abstract In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol). Porous scaffolds were obtained by freeze drying the combinations. The mechanical properties and in vitro growth of rat osteoblast‐like UMR‐106 cells were investigated. The investigation indicated that the compressive strength was controlled by the types of CaP. The highest compressive modulus of the composites was 479.77?MPa (23.84?MPa for compressive strength) which is for the BCPBG@ChiCol composite. Compressive modulus of 459.01 and 435.95?MPa with compressive strength of 22.73 and 17.89?MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively. In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface. Comparing the scaffolds, cells grown on the BCPBG based composite showed the higher cell density. To test its bioactivity, BCPBG@ChiCol was chosen for MTT and ALP assays on UMR‐106 cells. The results indicated that the UMR‐106 cells were viable and had higher ALP activity as the culturing times were increased. Therefore, ChiCol‐fabricated BCPBG scaffold shows promise for bone regeneration. ? 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1758–1766, 2017.
机译：摘要在本研究中，通过将三种不同磷酸钙（帽）（羟基磷灰石（HA），磷酸钙（TCP）和双相磷酸钙（BCP））混合的生物活性玻璃，通过浸入骨组织工程应用的支架进行（15CA：80SI：5P）（BG）用聚己内酯（PCL）作为壳聚糖/胶原（螯合）基质（CAPBG ZHICOL）的有机相中的粘合剂。通过冷冻干燥组合获得多孔支架。研究了大鼠成骨细胞样UMR-106细胞的机械性能和体外生长。调查表明，压缩强度由帽的类型控制。复合材料的最高压缩模量为479.77Ω·mPa（用于压缩强度的23.84mPa），其是BCPBG官方复合材料。 459.01和435.95的压缩模量分别具有22.73和17.89℃的压缩强度的MPa。体外细胞可用性和增殖试验证实了钙蛋白表面上的成骨细胞附着和生长。比较支架，基于BCPBG的复合材料上生长的细胞显示出更高的细胞密度。为了测试其生物活性，在UMR-106细胞上选择用于MTT和ALP测定的BCPBG Zicol。结果表明，随着培养时间增加，UMR-106细胞是可行的并且具有更高的ALP活性。因此，芝麻制造的BCPBG支架显示出骨再生的承诺。还2016 Wiley期刊，Inc.J生物保解员B：Appl Biomater，105B：1758-1766,2017。

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