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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Characterization of a biologically derived rabbit tracheal scaffold
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Characterization of a biologically derived rabbit tracheal scaffold

机译：生物衍生的兔气管脚手架的表征

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Abstract There is a clinical need to provide replacement tracheal tissue for the pediatric population affected by congenital defects, as current surgical solutions are not universally applicable. A potential solution is to use tissue engineered scaffold as the framework for regenerating autologous tissue. Rabbit trachea were used and different detergents (Triton x‐100 and sodium deoxycholate) and enzymes (DNAse/RNAse) investigated to create a decellularization protocol. Each reagent was initially tested individually and the outcome used to design a combined protocol. At each stage the resultant scaffold was assessed histologically, molecularly for acellularity and matrix preservation. Immunogenicity of the final scaffold was assessed by implantation into a rat model for 4?weeks. Both enzymes and detergents were required to produce a completely acellular (DNA content 42.78?ng/mg) scaffold with preserved collagen and elastin however, GAG content were reduced (8.78?±?1.35 vs. 5.5?±?4.8). Following in vivo implantation the scaffold elicited minimal immune response and showed significant cellular infiltration and vasculogenesis. The luminal aspect of the implanted scaffold showed infiltration of host derived cells, which were positive for pan cytokeratin. It is possible to create biologically derived biocompatible scaffolds to address specific pediatric clinical problems. ? 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2126–2135, 2017.
机译：摘要临床需要为受先天性缺陷影响的儿科人群提供替代气管组织，因为目前的手术解决方案不是普遍适用的。潜在的解决方案是使用组织工程脚手架作为再生自体组织的框架。使用兔气管和不同洗涤剂（Triton X-100和脱氧胆酸钠）和研究以产生脱细胞化方案的酶。每种试剂最初是单独测试的，并且用于设计组合方案的结果。在每个阶段，所得支架在组织学上进行评估，分子用于非细胞和基质保存。通过植入到大鼠模型中，评估最终支架的免疫原性4〜数周。需要两种酶和洗涤剂以生产完全无细胞（DNA含量42.78〜Ng / mg）支架，其具有保存的胶原蛋白和弹性蛋白，但是，降低了Gag含量（8.78？±1.35 Vs.5.5？±4.8）。遵循体内植入后，支架引起了极小的免疫应答，并显示出显着的细胞浸润和血管发生。植入的支架的腔体方面显示出宿主衍生细胞的浸润，这对于潘细胞角蛋白为阳性。可以创建生物学衍生的生物相容性支架以解决特定的小儿临床问题。还2016 Wiley期刊，INC.J生物保解率B部分：Appl Biomater，105B：2126-2135,2017。

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