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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Silk fibroin produced by transgenic silkworms overexpressing the Arg-Gly-Asp motif accelerates cutaneous wound healing in mice
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Silk fibroin produced by transgenic silkworms overexpressing the Arg-Gly-Asp motif accelerates cutaneous wound healing in mice

机译：通过转基因蚕产生的丝素蛋白过表达arg-gly-asp motif加速小鼠的皮肤伤口愈合

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摘要

We investigated the effect of silk fibroin (SF) on wound healing in mice. SF or an amorphous SF film (ASFF) prepared from silk produced by the wild-type silkworm Bombyx mori (WT-SF, WT-ASFF) or by transgenic worms that overexpress the Arg-Gly-Asp (RGD) sequence (TG-SF, TG-ASFF) was placed on 5-mm diameter full-thickness skin wounds made by biopsy punch on the back of 8-12 week-old BALB/c mice. Each wound was covered with WT-ASFF and urethane film (UF), TG-ASFF plus UF, or UF alone (control). Wound closure, histological thickness, the area of granulation tissue, and neovascularization were analyzed 4, 8, and 12 days later. The effect of SF on cell migration and proliferation was examined in vitro by scratch- and MTT-assay using human dermal fibroblasts. Wound closure was prompted by TG-ASFF, granulation tissue was thicker and larger in ASFF-treated wounds than the control, and neovascularization was promoted significantly by WT-ASFF. Both assays showed that SF induced the migration and proliferation of human dermal fibroblasts. The effects of TG-ASFF and TG-SF on wound closure, granulation formation, and cell proliferation were more profound than that of WT-ASFF and WT-SF. We document that SF accelerates cutaneous wound healing, and this effect is enhanced with TG-SF. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 97-103, 2019.
机译：我们研究了丝素蛋白（SF）对小鼠伤口愈合的影响。由野生型家蚕BOMBYX MORI（WT-SF，WT-ASFF）或通过过表达arg-Gly-ASP（RGD）序列的转基因蠕虫（TG-SF）的转基因蠕虫制备的SF或无定形SF膜（ASFF） TG-ASFF）被置于8毫米直径的全厚皮肤伤口，通过8-12周的BALB / C小鼠的后背进行活检冲头制成。将每个伤口覆盖有WT-ASFF和聚氨酯膜（UF），TG-ASFF加UF或单独的UF（对照）。伤口闭合，组织厚度，造粒组织面积和新生血管面积分析4,8和12天。通过使用人的皮肤成纤维细胞，通过刮擦和MTT测定检查SF对细胞迁移和增殖的影响。 TG-ASFF促使伤口闭合，肉芽组织较厚，在ASFF处理的伤口中比对照较大，并且通过WT-ASFF显着促进新血管形成。两种测定结果表明，SF诱导人皮肤成纤维细胞的迁移和增殖。 TG-ASFF和TG-SF对伤口闭合，造粒形成和细胞增殖的影响比WT-ASFF和WT-SF更深。我们记录了SF加速皮肤伤口愈合，并且通过TG-SF增强了这种效果。 （c）2018 Wiley期刊，Inc.J生物保解员B部分B：Appl Biomater，107B：97-103,2019。

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