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首页> 外文期刊>Journal of Biomolecular Structure and Dynamics >Fabrication of iron oxide nanocolloids using metallosurfactant-based microemulsions: antioxidant activity, cellular, and genotoxicity toward Vitis vinifera
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Fabrication of iron oxide nanocolloids using metallosurfactant-based microemulsions: antioxidant activity, cellular, and genotoxicity toward Vitis vinifera

机译：使用金属活性剂的微乳液制备氧化铁纳米胶体：抗氧化活性，细胞和遗传毒性对血管血管

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摘要

The present work aims at the fabrication of iron oxide nanocolloids using biocompatible microemulsion and their cytotoxic, genotoxic effect on Vitis vinifera plant has been evaluated. The three iron-based metallosurfactant complexes were synthesized. Nanosuspensions (Ns) were prepared using microemulsion technique and for the purpose, the microemulsion was prepared using oleic acid, butanol, tween 80 and as synthesized iron metallosurfactant. In this technique, no additional capping agent and/or reducing agent was added. Tween 80 which is a biocompatible surfactant acted as a reducing agent as well as stabilizing for the iron oxide Ns. Characterization of Ns's was done using TEM, FESEM, EDX, XRD, AFM, and zeta potential. Mixed type of iron oxide nanoparticles i.e. magnetite (Fe3O4), and maghemite (Fe2O3) with a size range of 1-16 nm was found to be present in the nanosuspensions prepared from all the three precursors. The antioxidant activity of the Fe Ns was also confirmed using DPPH assay, with order of activity FeDDA FeCTAC FeHEXA. The cellular toxicity of Ns was evaluated by observing the morphological changes on V. vinifera plant (petiole) using a light microscope. Further, the interactions of iron oxide Ns with V. vinifera's DNA (plant-DNA) was assessed using circular dichroism (CD) and gel electrophoresis. For the case of FeCTAC Ns, a decrease in the intensity of bands was observed indicating fragmentation or adduct formation resulting in DNA damage. In the case of FeDDA, a modest decrease in the intensity of bands was observed. However, for FeHEXA Ns, complete neutralization of bands was confirmed implying maximum damage to the plant DNA. CD, gel electrophoresis and antioxidant activity confirmed that FeHEXA Ns were most toxic and FeDDA Ns were safest among the three as-fabricated nanosuspensions.
机译：目前的作品旨在使用生物相容性微乳液和它们的细胞毒性，对血管毒性的遗传毒性作用进行了评估的氧化铁纳米核苷酸的制造。合成三种铁基金属活化剂配合物。使用微乳液技术制备纳米皂孔（NS），目的是使用油酸，丁醇，吐温80和合成铁金属活性剂制备微乳液。在该技术中，不加入额外的覆盖剂和/或还原剂。 TWEEN 80，其是生物相容性表面活性剂的作用为还原剂以及氧化氧化铁NS的稳定性。使用TEM，FESEM，EDX，XRD，AFM和Zeta电位表征NS的表征。发现含有1-16nm尺寸范围的磁铁矿纳米颗粒的混合型氧化铁纳米颗粒I.磁铁矿（Fe 3 O 4）和磁性镁料（Fe 2 O 3）存在于由所有三体制备的纳米杆子中存在。使用DPPH测定还使用DPPH测定来证实Fe NS的抗氧化活性，FEDDA＆GT的活性。 Fectac＆ Fehexa。通过使用光学显微镜观察V.Vinifera植物（叶柄）的形态变化来评估NS的细胞毒性。此外，使用圆形二色性（CD）和凝胶电泳评估氧化铁NS与V.Vinifera的DNA（植物DNA）的相互作用。对于Fectac NS的情况下，观察到带状强度的降低，表明碎裂或加合物形成导致DNA损伤。在FEDDA的情况下，观察到带强度的温度降低。然而，对于Fehexa NS，确认完全中和带暗示植物DNA的最大损伤。 CD，凝胶电泳和抗氧化活性证实，Fehexa NS最具毒，FedDA NS在三种制造的纳米杆菌中最安全。

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