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首页> 外文期刊>Journal of Camel Practice and Research >Using polyacryl amide gel electrophoresis for determination of serum and synovial fluid proteins in clinically healthy camels (Camelus dromedarius)
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Using polyacryl amide gel electrophoresis for determination of serum and synovial fluid proteins in clinically healthy camels (Camelus dromedarius)

机译：用聚丙烯酰胺凝胶电泳测定临床健康骆驼中血清和滑膜液蛋白（Camelus Dromedarius）

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摘要

Fifty adult one-humped Iranian camels (Camelus dromedarius) were studied to determine their protein components of synovial fluid and serum. All the animals were clinically normal with no clinical signs of locomotor disorders. Synovial fluid samples were taken from both elbow joints and blood samples were obtained from the jugular vein immediately prior to arthrocentesis. Protein components of synovial fluid and serum were studied by polyacryl amide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE). Electrophoretic patterns of synovial fluid and serum were similar and revealed 19 protein bands. Mean +- standard error of total protein in synovial fluid and serum were 27 +- 7 and 76 +- 8 g/L, respectively.
机译：研究了五十个成人一驼伊朗骆驼（Camelus Dromedarius），以确定其滑膜液和血清的蛋白质成分。 所有的动物在临床上正常，没有运动障碍的临床症状。 从肘关节中取出滑膜液样品，在节肢术之前立即从颈静脉获得血液样品。 通过在十二烷基硫酸钠（SDS-PAGE）存在下，通过聚丙烯酰胺酰胺凝胶电泳研究了滑膜液和血清的蛋白质组分。 滑膜液和血清的电泳模式类似且揭示了19个蛋白质带。 滑膜和血清中总蛋白质的平均±标准误差分别为27±7和76±8g / L.

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