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Protease-containing silicates as active antifouling materials

机译:含蛋白酶的硅酸盐作为活性防污材料

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Biocatalytic silicates,composite materials composed of #alpha#-chymotryps in and a silicate prepolymer,were prepared via a two-step polymerization process following solubilization of the enzyme in the polymerization media.This new approach resulted in active and stable composites,and a calculated half-life of over 350 days in aqueous buffer at 30degC.The high stability and activity of this biocatalytic silicate was likely ude to the covalent attachment between #alpha#-chymotrypsin and the silicate matrix.Theprotease-containing silicate was resistant to fouling by nonselective protein binding,as demonstrated by the dramatically reduced binding of human serum albumin to the silicate material when compared to that of a silicate containing pre-inactivated #alpha#-chymotrypsin.
机译:生物催化硅酸盐是由#alpha#-胰凝乳蛋白酶和硅酸盐预聚物组成的复合材料,是在酶在聚合介质中增溶之后,通过两步聚合过程制备的。这种新方法可产生活性和稳定的复合材料,并计算出在30°C的水性缓冲液中具有超过350天的半衰期。这种生物催化硅酸盐的高稳定性和活性可能归因于#α#-胰凝乳蛋白酶与硅酸盐基质之间的共价连接。含蛋白酶的硅酸盐能够抵抗非选择性污染如与含有预灭活的#α#-胰凝乳蛋白酶的硅酸盐相比,人血清白蛋白与硅酸盐材料的结合显着降低,证明了蛋白质结合。

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