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首页> 外文期刊>Journal of chemical neuroanatomy >Effects of maternal deprivation on the adrenocorticotrophic and gonadotrophic axes in the hypothalamo-pituitary unit of preweanling female sheep: the histomorphometric approach.
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Effects of maternal deprivation on the adrenocorticotrophic and gonadotrophic axes in the hypothalamo-pituitary unit of preweanling female sheep: the histomorphometric approach.

机译：孕产妇剥夺对肾上腺脊髓脊髓垂体单位的肾上腺皮肤病和促性营养轴的影响：组织形态近似法。

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This study was designed to investigate the histochemical effects of maternal deprivation on the adrenocorticotrophic and gonadotrophic axes in the hypothalamo-pituitary unit of preweanling lambs. Twelve-week-old female lambs were divided into either the control (lambs reared under undisturbed maternal conditions; n=3) or the maternally deprived group (lambs separated for three days from their dams; n=3). The corticotrophin-releasing hormone (CRH) and gonadotrophin-releasing hormone (GnRH) in the median eminence and the adenohypophyseal adrenocorticotrophin (ACTH), gonadotrophins (LH and FSH) and mRNAs for their beta-subunits were investigated using the immunohistochemistry or hybridohistochemistry. In maternally deprived lambs, the percentage of the area occupied by immunoreactive (ir)-CRH nerve terminals was lower (P<0.05) and the percentage of the adenohypophyseal area (PAA) occupied by ir-ACTH cells was higher (P<0.05) compared with the control lambs. In the hypothalamo-gonadotrophic axis of maternally deprived lambs the percentage of area occupied by ir-GnRH nerve terminals was higher (P<0.05) and the PAA occupied by ir-FSHbeta cells was lower (P<0.05) in comparison with controls. The PAA occupied by gonadotrophs detected using hybridohistochemistry was higher (P<0.05) for LHbeta-mRNA in contrast to a lower (P<0.05) percentage for FSHbeta-mRNA in maternally deprived lambs compared with those staying with dams. In conclusion, maternal deprivation affected the accumulation of CRH and ACTH. The different and more striking alterations in FSH synthesis and storage in comparison with those concerning LH were observed in maternally deprived lambs. Thus, rupture of the preweanling young-mother social contact can affect the gonadotroph population activity, especially that relating to FSH-producing cells in the infantile female sheep.
机译：本研究旨在探讨孕产妇剥夺对肾上腺素垂体单位的肾上腺皮质营养和促性腺营养轴的组织化学作用。将十二个母羊羔分为控制（在未受干扰的母体条件下饲养的羊羔; n = 3）或潜水剥夺的组（羊羔从水坝分开三天; n = 3）。使用免疫组织化学或杂交化学，研究了中位数和腺瘤肾上腺皮质养殖婴儿（ACTH），促性腺激素（LH和FSH）和MRNA的释放激素（CRH）和促性腺培养蛋白释放激素（GNRH）。在潜在的羊羔中，免疫反应性（IR）-crH神经终端占（P <0.05）占据的面积的百分比，IR-ACTH细胞占用的腺眼性面积（PAA）的百分比更高（P <0.05）与控制羔羊相比。在潜水缺失的羊草型促海拔植物轴上，IR-GNRH神经末端占据的面积的百分比越高（P <0.05），与对照相比，IR-FSHBETA细胞占据的PAA较低（P <0.05）。使用杂交化素检测到的促性腺素占据的PAA对于LHBETA-mRNA的较高（P <0.05），与潜水艇的羊皮纸中的FSHBETA-mRNA的较低（P <0.05）百分比相比，与水坝保持相比。总之，母体剥夺影响了CRH和ACTH的积累。在母产物剥夺的羊羔中观察到与LH有关LH的储存不同和更醒目的改变。因此，屈服的年轻母亲社交接触的破裂可能会影响促进的促性腺群体活动，特别是与婴儿女性绵羊的生成细胞有关的促性腺群体活动。

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