...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of chemical neuroanatomy >Calbindin immunostaining in the CA1 hippocampal pyramidal cell layer of the human and mouse: A comparative study
【24h】

Calbindin immunostaining in the CA1 hippocampal pyramidal cell layer of the human and mouse: A comparative study

机译：CA1海马锥体细胞层的CALBINDIN免疫染色人和小鼠：比较研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Immunostaining for calbindin (CB) is commonly used to label particular populations of neurons. Recently, it has been shown that the CA1 pyramidal cells in the mouse can be subdivided along the radial axis into superficial and deep pyramidal cells and that this segregation in the radial axis may represent a general principle of structural and functional organization of the hippocampus. One of the most widely used markers of the superficial pyramidal cells is CB. However, this laminar segregation of pyramidal cells has not been reported in the human CA1 using CB immunostaining. The problem is that the different pattern of CB immunostaining observed in the mouse compared to the human could be explained by technical features, of which one of the most important is the postmortem time (PT) delay typical of the brain tissue obtained from humans. In the present study, we have studied the influences of PT delays and fixation procedures and we found that the clear differences found between the CA1 of the human and mouse do not depend on the fixation, but represent actual species-specific differences. These remarkable differences between species should be taken into consideration when making interpretations in translational studies from mouse to human brains.
机译：Calbindin（CB）的免疫染色通常用于标记神经元的特定群体。最近，已经表明，小鼠中的Ca1金字塔孔细胞可以沿径向轴细分成浅表和深锥体细胞，并且在径向轴上的这种隔离可以代表海马的结构和功能组织的一般原理。浅表锥形锥形细胞的最广泛使用的标记之一是CB。然而，使用Cb免疫染色的人CA1尚未在人CA1中报道该层状细胞的这种层状偏析。问题是，与人类相比，在鼠标中观察到的CB免疫抑制的不同模式可以通过技术特征来解释，其中最重要的是从人类获得的脑组织的典型后期时间（PT）延迟之一。在本研究中，我们研究了PT延迟和固定程序的影响，我们发现人和小鼠的CA1之间发现的明显差异不依赖于固定，而是代表实际特异性差异。当在从小鼠到人性大脑中的翻译研究中解释时，应考虑物种之间的这些显着差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号