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首页> 外文期刊>Journal of chemical neuroanatomy >Cytoplasmic distribution of T-box transcription factor Tbr-1 in adult rodent brain.
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Cytoplasmic distribution of T-box transcription factor Tbr-1 in adult rodent brain.

机译：成人啮齿动物脑中T型转录因子TBR-1的细胞质分布。

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摘要

T-brain-1 (Tbr-1), a brain-specific T-box transcription factor, plays a critical role in cerebral cortex and olfactory bulb development. The expression levels of Tbr-1 are highest at the embryonic stage and are gradually reduced during the developmental process. In adult brain, Tbr-1 is expressed at a lower, but still significant level. Tbr-1 transcriptional activity is enhanced via interaction with CASK, a membrane-associated guanylate kinase, but it is not clear whether any other mechanism regulates Tbr-1 activity. We examined the subcellular distribution of Tbr-1 in adult and postnatal rat brains using DAB stain and confocal imaging analysis. In contrast to the embryonic stage, Tbr-1 was distributed in P3 and adult rat brain in the nucleus as well as the cytoplasm of neurons in the cerebral cortex and hippocampus. Confocal analysis clearly showed dendritic distribution of Tbr-1 in pyramidal neurons. In the cerebellum of P15, P22, and adult rats, Tbr-1 was specifically expressed in Purkinje cells, where Tbr-1 was localised in the cytoplasm, including the dendritic tree. In addition, biochemical fractionation of adult cerebral cortex and hippocampus showed that cytoplasmic Tbr-1 is highly enriched in the lysed synaptosomal fraction, further indicating a synaptic distribution of cytoplasmic Tbr-1 in adult brain. Our study suggests that translocation from synapse to the nucleus is involved in regulation of Tbr-1 function in postnatal and adult brains.
机译：T-Brain-1（TBR-1），脑特异性T型箱转录因子，在脑皮质和嗅灯泡发育中起着关键作用。 TBR-1的表达水平在胚胎阶段最高，并且在发育过程中逐渐减少。在成人脑中，TBR-1表示在较低，但仍然仍然存在显着水平。通过与桶的相互作用，膜相关的胍基激酶的相互作用来增强TBR-1转录活性，但目前尚不清楚是否有任何其他机制调节TBR-1活性。我们使用DAB染色和共焦成像分析检查了成人和产后大鼠大脑中TBR-1的亚细胞分布。与胚胎阶段相比，TBR-1分布在核中的p3和成年大鼠脑中，以及脑皮质和海马中神经元的细胞质。共聚焦分析清楚地显示了金字塔神经元中TBR-1的树突分布。在P15，P22和成年大鼠的小脑中，TBR-1在purkinje细胞中特异性地表达，其中TBR-1在细胞质中定位，包括树突树。此外，成人脑皮质和海马的生化分馏表明，细胞质TBR-1在裂解的突触体馏分中高度富集，进一步表明成人脑中细胞质TBR-1的突触分布。我们的研究表明，从核的突触转向涉及产后和成人大脑中TBR-1功能的调节。

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