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首页> 外文期刊>Journal of chemical neuroanatomy >The petrosal vein mutilation affects the SOD activity, MDA levels and AQP4 level in cerebellum and brain stem in rabbit
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The petrosal vein mutilation affects the SOD activity, MDA levels and AQP4 level in cerebellum and brain stem in rabbit

机译：岩石静脉残留对小脑和脑干中的草皮活性，MDA水平和AQP4水平影响兔子

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Cerebral edema after brain surgery remains a life-threatening complication in the clinic. For a better operating field view, superior petrosal vein (SPV) can be easily damaged during neurosurgery. SPV sacrifice may sometimes be inevitable in clinic. However, the safety of SPV sacrifice is still a controversial question. Whether petrosal vein injury has an effect on cerebral edema after brain surgery is still unknown. In this study, rabbits were divided into two groups. The rabbits in the surgery group underwent petrosal vein sacrifice. The control group was subjected to sham surgery. Cerebellum and brain stem tissues were collected at 4 h, 8 h, 12 h, 24 h, 48 h and 72 h post-surgery. The superoxidase dismutase (SOD) activity and expression of malondialdehyde (MDA) were tested in the collected samples. Quantitiative real time polymerase chain reaction and immunohistochemistry were used to detect the mRNA and protein levels, respectively, of aquaporin 4 (AQP4) in the tissue samples. Compared to the control sham group, the activity of SOD and MDA expression in cerebellum was decreased and increased, respectively, at 4 h, 8 h, 12 h and 24 h post-, surgery The SOD activity and expression of MDA in brain stem was decreased and increased, respectively, only in 4 h after surgery, compared with control group. The mRNA and protein levels of AQP4 were increased in cerebellum at 4 h, 8 h, 12 h and 24 h after surgery, but in the brain stem, the levels were increased only at 4 h after surgery compared with sham group. Our results thus show that SPV sacrifice influences oxidative stress and the expression of AQP4 in cerebellum and brain stem of rabbits; highlighting the importance of protecting the petrosal vein during neurosurgery.
机译：脑外手术后脑水肿仍然是临床危及生命的并发症。为了更好的操作场景，在神经外科术期间可以容易地损坏优良的浮子静脉（SPV）。 SPV牺牲有时可能在诊所不可避免。然而，SPV牺牲的安全仍然是一个有争议的问题。瘀岩静脉损伤是否在脑外手术仍然未知后对脑水肿有影响。在这项研究中，兔子分为两组。手术组中的兔子接受了岩石静脉牺牲。对照组进行假手术。在手术后4小时，8小时，12小时，24小时，48小时和72小时收集细胞脑和脑干组织。在收集的样品中测试了超氧化酶歧化酶（SOD）活性和丙二醛（MDA）的表达。定量实时聚合酶链反应和免疫组织化学分别用于在组织样品中分别检测水素4（AQP4）的mRNA和蛋白水平。与对照Sham组相比，小脑中的SOD和MDA表达的活性分别下降，分别在4小时，8小时，12小时和24小时，手术治疗SOD活性和MDA在脑干中的表达是与对照组相比，分别在手术后的4小时内降低和增加。在手术后4小时，8小时，12小时和24小时的小脑中，AQP4的mRNA和蛋白质水平增加，但在脑干中，与假组合相比，手术后4小时，水平仅增加水平。因此，我们的结果表明，SPV牺牲影响氧化应激和AQP4在小脑中的表达和兔的脑干;突出了神经外科术治疗岩石静脉的重要性。

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