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首页> 外文期刊>Journal of Comparative Pathology >Evaluation of Cytopathological Techniques for the Diagnosis of Canine Visceral Leishmaniosis with Lymph Node Samples
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Evaluation of Cytopathological Techniques for the Diagnosis of Canine Visceral Leishmaniosis with Lymph Node Samples

机译：淋巴结样品诊断犬内脏利伊氏病的缩细胞病理学技术评价

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摘要

The identification of the parasite in cytological smears of lymph node aspirates is a widely applied technique for the direct diagnosis of Leishmania spp. infection, especially in endemic areas. Although very specific, this method has limited sensitivity, and improving the technique would be highly desirable. This study aimed to evaluate the efficacy of conventional smear cytology (SC), liquid-based cytology (LBC), cell block (CB) stained with haematoxylin and eosin (HE) and immunocytochemistry (ICC), and formalin-fixed paraffin wax-embedded tissue immunohistochemistry (FFPE-IHC) compared with serology and polymerase chain reaction for the diagnosis of canine visceral leishmaniosis (CVL) in lymphoid tissue. The use of a preservative medium and centrifugation for cytological samples reduced the number of unsatisfactory artefacts/background. Moreover, LBC allowed excellent cellular preservation and the application of ancillary techniques, such as CB and ICC. SC was the most accurate morphological diagnostic method (45.0%). CB-ICC alone or associated with SC demonstrated significantly higher sensitivity (70.0% and 72.0%, respectively) when compared with SC alone (34.00%). CB-ICC was found to be more effective in the detection of infected animals with mild clinical signs, similar to FFPE-IHC. The specificity and positive predictive value were similar between all methods. Finally, the detection limit for CB-ICC and SC + CB-ICC was identical (18.46 amastigotes/ mm(2)). Our study suggests that CB-ICC is a promising tool for improvement of the cytopathological diagnosis of CVL and may be applied in routine epidemiological screening. (C) 2019 Elsevier Ltd. All rights reserved.
机译：寄生虫在淋巴结吸气中的细胞学涂片中的鉴定是对LeishMania SPP直接诊断的广泛应用技术。感染，尤其是地方性区域。虽然非常具体，但这种方法的灵敏度有限，并且改善了该技术将是非常理想的。本研究旨在评估常规涂抹细胞学（SC），液体基细胞学（LBC），细胞嵌段（CB），用血红素和曙红（HE）和免疫细胞化学（ICC），以及福尔马林固定石蜡嵌入的疗效组织免疫组织化学（FFPE-IHC）与血清学和聚合酶链反应进行淋巴组织中犬内脏Leishmaniosis（CV1）的血清学和聚合酶链反应。使用防腐培养基和离心用于细胞学样品，减少了不令人满意的人工制品/背景的数量。此外，LBC允许优异的细胞保存和辅助技术的应用，例如CB和ICC。 SC是最准确的形态诊断方法（45.0％）。与单独的SC相比，单独或与SC相关的CB-ICC分别显示出显着更高的敏感性（分别为70.0％和72.0％）（34.00％）。发现CB-ICC在检测具有轻度临床症状的感染动物中更有效，类似于FFPE-IHC。所有方法之间的特异性和阳性预测值相似。最后，CB-ICC和SC + CB-ICC的检测限相同（18.46Amastigotes / mm（2））。我们的研究表明，CB-ICC是提高CVL细胞病理学诊断的有希望的工具，并且可以应用于常规流行病学筛查。 （c）2019年elestvier有限公司保留所有权利。

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  • 来源
    《Journal of Comparative Pathology》 |2019年第2019期|共10页
  • 作者

    Guerr J. M.; Fernandes N. C. C. A.; Ressio R. A.; Magno J. A.; Kimura L. M.; Barbosa J. E. de R.; Bertollo D. M. B.; Taniguchi H. H.; Hiramoto R. M.; Motoie G.; Tolezano J. E.; Cogliati B.;

  • 作者单位

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Secretaria Estado Saude Sao Paulo Coordenadoria Controle Doencas Ctr Lab Reg Inst Adolfo Lutz Sao Jose do Rio Pret Rua Alberto Sufredini Bertoni 2325 Sao Jose Do Rio Preto SP Brazil;

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Univ Sao Paulo Coordenadoria Controle Doencas Secretaria Estado Saude Sao Paulo Inst Adolfo Lutz Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

    Univ Sao Paulo Dept Patol Fac Med Vet &

    Zootecnia Av Prof Orlando Marques de Paiva 87 Sao Paulo Brazil;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 病理学;
  • 关键词

    cytology; dog; immunocytochemistry; Leishmania;

    机译：细胞学;狗;免疫细胞化学;Leishmania;

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