...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of clinical laboratory analysis. >Evaluation of the RT RT ‐ LAMP LAMP and LAMP LAMP methods for detection of Mycobacterium tuberculosis Mycobacterium tuberculosis
【24h】

Evaluation of the RT RT ‐ LAMP LAMP and LAMP LAMP methods for detection of Mycobacterium tuberculosis Mycobacterium tuberculosis

机译：评价RT RT灯灯和灯泡灯方法检测分枝杆菌分枝杆菌结核分枝杆菌

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The current methods for detecting Mycobacterium tuberculosis (Mtb) are not clinically optimal. Standard culture methods ( SCM s) are slow, costly, or unreliable, and loop‐mediated isothermal amplification ( LAMP ) cannot differentiate live Mtb. Methods This study compared reverse transcription ( RT )‐ LAMP , LAMP , and an SCM for detecting Mtb. A first experiment tested the sensitivity and specificity of primers for 9 species of Mycobacterium (H37Rv, M. intracellulare , M. marinum , M. kansasii , M. avium , M. flavescens , M. smegmatis , M. fortuitum , and M. chelonae ); and 3 non‐Mycobacterium species (Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae). A second experiment tested sputum specimens for the presence of Mtb, from 100 patients with tuberculosis (clinical) and 22 from patients without tuberculosis (control), using Roche solid culture ( SCM ), LAMP , and RT ‐ LAMP . In the clinical samples. Results The rates of positivity for Mtb of the SCM , LAMP , and RT ‐ LAMP methods were 88%, 92%, and 100%, respectively. The difference in detection rate was significant between RT ‐ LAMP and SCM , but RT ‐ LAMP and LAMP were comparable. In the control group, the detection rates were nil for all three methods. Conclusion The specificities of the methods were similar. The sensitivity of RT ‐ LAMP was ~10‐fold higher than that of LAMP for detecting Mtb. Unlike LAMP , RT ‐ LAMP could identify viable bacteria, and was able to detect a single copy of Mtb. Among SCM , LAMP , and RT ‐ LAMP , the latter is the most suitable for wide use in the lower‐level hospitals and clinics of China for detecting Mtb in sputum samples.
机译：背景技术检测分枝杆菌（MTB）的目前的方法在临床上不是最佳的。标准培养方法（SCM S）缓慢，昂贵或不可靠，环介导的等温扩增（灯）不能区分Live MTB。方法对比较逆转录（RT）灯，灯，灯和用于检测MTB的SCM。第一个实验测试了9种分枝杆菌的引物的敏感性和特异性（H37RV，M.骨髓内部，M.Marinum，M.Kansasii，M.Avium，M.Flavescens，M. Smogmatis，M.Fortuitum和M. Chelonae ）;和3种非分枝杆菌（金黄色葡萄球菌，假单胞菌，铜绿假单胞菌和Klebsiella肺炎）。第二个实验测试了MTB存在的痰标本，从100名结核病（临床）和22例，没有结核病（对照），使用Roche固体培养（SCM），灯和RT灯。在临床样本中。结果SCM，灯和RT灯方法的MTB阳性率分别为88％，92％和100％。 RT灯和SCM之间检测速率的差异显着，但RT - 灯和灯可相当。在对照组中，所有三种方法的检测率为NIL。结论该方法的特异性相似。 RT - 灯的灵敏度高于用于检测MTB的灯的10倍。与灯不同，RT - 灯可以识别可行的细菌，并且能够检测到单一的MTB副本。在SCM，灯和RT灯中，后者最适合在中国较低级别的医院和诊所用于检测痰样品中的MTB。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号