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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Development of a high-performance liquid chromatography method for the determination of florfenicol in animal feedstuffs
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Development of a high-performance liquid chromatography method for the determination of florfenicol in animal feedstuffs

机译：高效液相色谱法测定动物饲料中氟苯酚的高效液相色谱法

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Highlights?A TLC-HPLC method was developed for determining florfenicol in animal feedstuffs.?The analytical method was proved to be reliable, simple and cost-effective.?TLC could be used as an alternative purification technique for complex matrix.AbstractAn effective thin layer chromatography (TLC) purification procedure coupled to high-performance liquid chromatography (HPLC) method was developed for the determination of florfenicol (FF) in pig, chicken and fish feedstuffs. The feedstuff samples were extracted with ethyl acetate, defatted with n-hexane saturated with acetonitrile, and further purified by TLC. The chromatographic separation was performed on a Waters Symmetry C18column using an isocratic procedure with acetonitrile-water (35:65, v/v) at 0.6mL/min. The ultraviolet (UV) detector was set at a wavelength of 225nm. The FF concentrations in feedstuff samples were quantified using a standard curve. Good linear correlations (y=159075x
机译：<！[cdata [ 突出显示 ？ 开发了TLC-HPLC方法，用于测定动物饲料中的弗洛芬醇。 < CE：list-item id =“lsti0010”> ？ 证明分析方法是可靠的，简单和成本- ？ TLC可用作复杂矩阵的替代净化技术。 摘要 一种有效的薄层色谱（ TLC）纯化方法与高效液相色谱（HPLC）方法偶联，用于测定猪，鸡肉和鱼类饲料中的氟苯纤维（FF）。用乙酸乙酯萃取饲料样品，用饱和乙腈饱和的正己烷脱落，并通过TLC进一步纯化。使用具有乙腈 - 水（35:65，v / v）的异形方法在0.6ml / min时进行色谱分离在水对称性c 柱中进行色谱分离。 。紫外线（UV）检测器设定为225nm的波长。使用标准曲线量化FeedStuff样品中的FF浓度。良好的线性相关性（ y = 159075 x

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