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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Quantification of ondansetron, granisetron and tropisetron in goat plasma using hydrophilic interaction liquid chromatography-solid phase extraction coupled with hydrophilic interaction liquid chromatography-triple quadrupole tandem mass spectrometry
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Quantification of ondansetron, granisetron and tropisetron in goat plasma using hydrophilic interaction liquid chromatography-solid phase extraction coupled with hydrophilic interaction liquid chromatography-triple quadrupole tandem mass spectrometry

机译:使用亲水性相互作用液相色谱 - 固相萃取与亲水相互作用液相色谱 - 三重四极杆串联质谱 - 三重四极杆串联质谱法,定量山羊等离子体中的山羊等离子体液相色谱 - 固相萃取

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摘要

An assay method to quantify ondansetron (OND), granisetron (GRA) and tropisetron (TRO) in goat plasma has been successfully developed and validated. This method procedure for the analysis of OND, GRA and TRO was involved of extracting samples with hydrophilic interaction liquid chromatography (HILIC) solid phase extraction (SPE) and determination by liquid chromatography coupled to tandem mass spectroscopy. An SPE method for the simultaneous extraction of OND, GRA and TRO with high efficiency and selectivity was developed. Prior to HPLC-MS/MS analysis, most of the sources of interference present in the supernatant after protein precipitation of plasma proteins was efficiently removed from the samples by the HILIC SPE treatment. For the quantification of OND, GRA and TRO in the samples, tandem mass spectrometry operating in positive electrospray ionization mode with multiple reaction monitoring was used. The calibration curve was performed in the range of 0.2–20?ng/mL for the target OND, GRA and TRO in goat plasma samples. The precision of the intra- and inter-day assay for OND, GRA and TRO were 1.84–6.23% and 3.89–5.31%, 2.63–6.29% and 3.76–5.31%, 1.99–5.67% and 2.64–4.70%, respectively. The accuracy of the intra- and inter-day assay for OND, GRA and TRO were 89.15–97.39% and 89.46–95.17%, 91.08–100.82% and 91.24–99.47%, 92.30–100.74% and 94.21–97.90%, respectively. For the determination of OND, GRA and TRO in plasma samples, no significant matrix effects were observed. The mean absolute recoveries were 103–150%, 115–121%, and 98–141% for OND, GRA and TRO, respectively. Furthermore, the mean process efficiency values of silica SPE were 98–135%, 92–124%, and 72–109% for OND, GRA and TRO, respectively.
机译:已经成功开发并验证了山羊等离子体中量化ondansetron(OND),Granisetron(GRA)和对象菌素(Trop)的测定方法已经成功开发和验证。该方法用于分析IND,GRA和TRO的方法涉及用亲水性相互作用液相色谱(HILIC)固相萃取(SPE)提取样品,并通过液相色谱偶联与串联质谱法测定。开发了具有高效率和选择性的同时提取OND,GRA和TR的SPE方法。在HPLC-MS / MS分析之前,通过HILIC SPE处理有效地从样品中获得蛋白质蛋白质蛋白质沉淀后上清液中存在的大多数干扰源。为了定量在样品中的OND,GRA和TRO,使用在具有多重反应监测的正电喷雾电离模式下进行的串联质谱。校准曲线在山羊等离子体样品中的目标IND,GRA和TR的0.2-20℃/ ml的范围内进行。 OND,GRA和TR的日期和白天测定的精确度分别为1.84-6.23%和3.89-5.31%,2.63-6.29%和3.76-5.31%,分别为1.99-5.67%和2.64-4.70%。 OND,GRA和TR的日间测定的准确性分别为89.15-97.39%和89.46-95.17%,91.08-100.82%和91.24-99.47%,92.30-100.74%和94.21-97.90%。为了测定血浆样品中的IND,GRA和TRA,没有观察到显着的基质效应。 IND,GRA和TRO的平均绝对回收率分别为103-150%,115-121%和98-141%。此外,二氧化硅SPE的平均工艺效率值分别为OND,GRA和TR的98-135%,92-124%和72-109%。

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  • 作者单位

    Faculty of Chemical Environmental and Biological Science and Technology Analytical Center Dalian University of Technoloy;

    Faculty of Chemical Environmental and Biological Science and Technology Analytical Center Dalian University of Technoloy;

    Faculty of Chemical Environmental and Biological Science and Technology Analytical Center Dalian University of Technoloy;

    Faculty of Chemical Environmental and Biological Science and Technology Analytical Center Dalian University of Technoloy;

    Department of Radiation Oncology Second Affiliated Hospital of Dalian Medical University;

    Faculty of Chemical Environmental and Biological Science and Technology Analytical Center Dalian University of Technoloy;

    School of Pharmaceutical Science and Technology Dalian University of Technology;

    Faculty of Chemical Environmental and Biological Science and Technology Analytical Center Dalian University of Technoloy;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学技术;
  • 关键词

    Ondansetron; Granisetron; Tropisetron; HILIC-SPE; HILIC-MS/MS; Plasma;

    机译:ondansetron;granisetron;tropisetron;hilic-spe;hilic-ms / ms;血浆;

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