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首页> 外文期刊>Journal of Economic Entomology >Identification and Distribution of Haplotypes of Diaphorina citri (Hemiptera: Liviidae) in Jamaica and the Caribbean
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Identification and Distribution of Haplotypes of Diaphorina citri (Hemiptera: Liviidae) in Jamaica and the Caribbean

机译：牙买加和加勒比地区康乃狄纳Citri（Hemiptera：Liviidae）鉴定和分布

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摘要

Diaphorina citri Kuwayama (Hemiptera: Psyllidae), also known as the Asian citrus psyllid, is a vector of the citrus huanglongbing (HLB) disease. D. citritransmits all three known strains of the HLB pathogen: Candidatus Liberibacter africanus, Candidatus Liberibacter americanus, and Candidatus Liberibacter asiaticus.The study involved 92 psyllids representing the Caribbean Basin and reference samples representing countries within Asia, North America, and South America. This study was aimed at characterizing D. citri on a molecular level in order to determine the haplotype diversity and uniqueness within Jamaica and the Caribbean Basin. D. citri-specific primers were used to amplify an 821 bp gene fragment from the mitochondrial cytochrome c oxidase subunit I gene (mtCOI). The statistical parsimony program, TCS, was used to determine the 12 haplotypes found within the Caribbean, with haplotypes H2 and H7 being the most prominent.The H2 haplotype was found to belong to the South Western Asia group originating from India. H2 represented 54% of the sequenced samples and dominated the Greater Antilles, 22% were grouped as H7, dominating the Lesser Antilles, while the remaining 24% of the sequences were grouped in the remaining 10 haplotypes and were variants seen within the Greater and Lesser Antilles.
机译：Diaphorina Citri Kuwayama（Hemiptera：psyllidae），也称为亚洲柑橘类氏植物，是柑橘黄龙（HLB）疾病的载体。 D.鉴定拟已知的HLB病原体菌株：Candidatus Libibacter AfforaNus，Candidatus Libibacter Americanus，以及Candidatus Libibacter Asiaticus。研究涉及占亚洲，北美和南美洲内部的加勒比流域和参考样本的92个百辉莲花疟原虫。本研究旨在表征分子水平的D. Citri，以确定牙买加和加勒比盆地的单倍型多样性和独特性。 D. Citri特异性引物用于扩增来自线粒体细胞色素C氧化酶亚基I基因的821bp基因片段（MTCOI）。统计分析程序TCS用于确定加勒比海内发现的12个单倍型，具有单倍型H2和H7是最突出的。发现H2单倍型属于来自印度的南方亚洲组。 H2代表54％的测序样品并主导更大的安妥尔葡萄酒，22％被分组为H7，占较小的安妥尔斯，而剩余的24％的序列在其余10个单倍型中被分组，并且在较小和较小的剩余物质中被分组。安妥尔太。

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